Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

P4860

Sigma-Aldrich

Protease from Bacillus licheniformis

≥2.4 U/g

Synonym(s):

Alcalase® 2.4L, Proteinase from Bacillus licheniformis, Subtilisin A

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9014-01-1
Enzyme Commission number:
3.4.21.62 (BRENDA, IUBMB)
EC Number:
232-752-2
MDL number:
MFCD00132092
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Pricing and availability is not currently available.

form

aqueous solution

Quality Level

200

specific activity

≥2.4 U/g

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.[1]

Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations.
The enzyme from Sigma has been used to prepare chicken (leg) bone protein hydrolysates while evaluating ACE inhibitory peptides.[2] It has also been used for the hydrolysis of Chlorella pyrenoidosa protein extracts during a study of their antitumor activities.[3]

Biochem/physiol Actions

Alcalase is an endo-protease of the serine type. It has a broad substrate specificity and can hydrolyze most peptide bonds within a protein molecule. It is active between pH 6.5 and 8.5 and has an optimum temperature of 60 °C. The enzyme is is used in detergent formulations to remove protein-based stains.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Analysis Note

Activity expressed in Anson Units

Legal Information

A product of Novozyme Corp.
Alcalase is a registered trademark of Novozymes Corp.

Signal Word

Danger

Hazard Statements

H315,H318,H334,H335,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000409449
0000409449
0000404725
0000383620
0000378840

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Andrew R Hirst et al.
Nature chemistry, 2(12), 1089-1094 (2010-11-26)
Supramolecular gels, which demonstrate tunable functionalities, have attracted much interest in a range of areas, including healthcare, environmental protection and energy-related technologies. Preparing these materials in a reliable manner is challenging, with an increased level of kinetic defects observed at
Srinidhi Jayaraman et al.
The Journal of pharmacology and experimental therapeutics, 379(2), 191-202 (2021-08-15)
Neurolysin (Nln) is a recently recognized endogenous mechanism functioning to preserve the brain from ischemic injury. To further understand the pathophysiological function of this peptidase in stroke and other neurologic disorders, the present study was designed to identify small molecule
Xiaoqin Wang et al.
Biotechnology progress, 29(3), 681-687 (2013-04-23)
Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion
Proteases: multifunctional enzymes in life and disease
Lopez-Otin C and Bond JS
Test, 283(45), 30433-30437 (2008)
Catherine Cesa-Luna et al.
PloS one, 15(10), e0240545-e0240545 (2020-10-16)
The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production
View All Related Papers

Questions

1–10 of 12 Questions  

  1. Is this a food grade product?

    1 answer

    1. This product is intended for research use only and is not suitable for human or veterinary use. Currently, a food grade product is not available.

      Helpful?

  2. What is the density of the P4860 enzyme?

    1 answer

    1. This item is a product of Novozymes Corp. The supplier reports a density of 1.17g/ml at 20 degrees C.

      Helpful?

  3. What is optimal temperature and ph for this enzyme?

    1 answer

    1. According to the manufacturer’s (Novozymes Corp.) Product Sheet, by analyzing Alcalase at various pH values using a modified Anson/haemoglobin method, its optimum activity has been found to be at pH between 6.5 and 8.5; its temperature optimum is around 60°C, but could vary between 55°C and 70°C, depending on the type of substrate.

      Helpful?

  4. Can this be used for production of fish protein hudrolysate? Also what is the difference between this enzymeand Alcalse 2.4l?

    1 answer

    1. This product is identical to Alcalase 2.4L. This material has not been tested for use in fish. A search of the literature shows a number of publications related to the use of this enzyme in animal protein production. However, a specific reference for use in fish was not found. Please see the links below to review articles that may be of interest:

      Chicken meat
      https://ift.onlinelibrary.wiley.com/doi/10.1111/j.1750-3841.2008.00765.x

      Fish scales
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063752/

      Helpful?

  5. What is the extinction coefficient?

    1 answer

    1. The extinction coefficient is not determined for this product.

      Helpful?

  6. What is the extinction coefficient for this enzyme?

    1 answer

    1. The extinction coefficient for this enzyme is not determined.

      Helpful?

  7. What is the molecular weight?

    1 answer

    1. The molecular weight of Protease from Bacillus licheniformis (Product No. P4860) is approximately 27 kDa.

      Helpful?

  8. What is the optimal pH for the use of enzyme P4860?

    1 answer

    1. According to the manufacturer Novozyme, the optimal pH for the use of product P4860 is approximately pH 9.

      Helpful?

  9. What is the concentration of the product, and how can the conversion from units/g on the COA to units/ml be performed?

    1 answer

    1. The density of the Novozymes product, as indicated in the SDS sheet, is 1.17g/ml. By multiplying 1.17 g/ml (density) by 50 ml (pack size), 58.5 g of the solution is obtained. Further multiplication with 2.55 unit/g (lot activity) results in 149.175 or 149.2 units. Dividing 149.2 units by 50ml gives 2.984 units/ml.

      Helpful?

  10. Does this protease contain any α-amylase or amyglucosidase?

    1 answer

    1. Whether this product contains α-amylase or amyglucosidase is not determined.

      Helpful?

1–10 of 12 Questions  

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service