Mero-25

9100102, human lung, Mixed epithelial and spindle shaped

• UNSPSC Code: 41106514

Properties

• product name: Mero-25, 09100102
• biological source: human lung
• growth mode: Adherent
• karyotype: Number of chromosomes 127-140
• morphology: Mixed epithelial and spindle shaped
• products: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Cell Line Description

Mero-25 was derived from autopsy tumour material from a male patient aged >60 with a history of asbestos exposure

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 11,12
D7S820: 9
THO1: 8
TPOX: 9
vWA: 16,17

Culture Medium

Hams F10 + 15% FCS + 2mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 48 hours

Other Notes

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