Mero-48a

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 9100104, human lung, Mixed epithelial and spindle shaped

• UNSPSC Code: 41106514
• Biological source: human lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Number of chromosomes 71-75
• Morphology: Mixed epithelial and spindle shaped

Properties

• biological source: human lung
• packaging: tube of 5 μg 09100104-DNA-5UG, pkg of vial of cells 09100104-1VL
• growth mode: Adherent
• karyotype: Number of chromosomes 71-75
• morphology: Mixed epithelial and spindle shaped
• products: Epithelial membrane antigen (EMA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Biochem/physiol Actions

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Mero-48a was derived from autopsy tumour material from the pleural cavity

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9,11
D5S818: 11,12
D7S820: 9,10
THO1: 9.3
TPOX: 8
vWA: 17,18

Preparation Note

Hams F10 + 15% FCS + 2mM Glutamine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24 hours

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].