The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.
565325-U
Ascentis® RP-Amide (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonyme(s) :
RP-Amide Reversed-Phase Chromatography Column
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About This Item
Code UNSPSC :
41115700
eCl@ss :
32110501
Nomenclature NACRES :
SB.52
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Nom du produit
Colonne HPLC Ascentis® RP-Amide, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Matériaux
stainless steel column
Agence
suitable for USP L60
Gamme de produits
Ascentis®
Caractéristiques
endcapped
Fabricant/nom de marque
Ascentis®
Conditionnement
1 ea of
Ampleur du marquage
19.5% Carbon loading
Paramètres
≤70 °C temp. range
400 bar pressure (5801 psi)
Technique(s)
HPLC: suitable
LC/MS: suitable
L × D.I.
25 cm × 4.6 mm
Superficie
450 m2/g
Couverture de surface
2.7 μmol/m2
Impuretés
<5 ppm metals
Matrice
fully porous particle
silica gel high purity, spherical
Groupe de la matrice active
amide, alkyl phase
Taille des particules
5 μm
Dimension de pores
100 Å
Plage de pH de fonctionnement
2-8
Application(s)
environmental
food and beverages
Technique de séparation
reversed phase
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Catégories apparentées
Description générale
Ascentis RP-Amide est une phase EPG (groupement polaires inclus) de nouvelle génération à faible relargage, qui fournit une sélectivité orthogonale et une plus grande résolution pour l′analyse des composés polaires en HPLC et LC-MS. L′Ascentis RP-Amide la phase de première intention à groupement polaire incorporé pour HPLC.
Application
- A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study presents a validated Ultra-High-Performance Liquid Chromatography (UHPLC) method employing an RP-Amide fused-core column for the quantitative determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The method demonstrated excellent precision, accuracy, and sensitivity, making it a valuable tool for the analysis of these bioactive compounds in complex matrices. The research highlights the utility of the Ascentis® RP-Amide HPLC Column in enhancing the resolution and efficiency of chromatographic separations in analytical chemistry applications (Fibigr et al., 2018).
Caractéristiques et avantages
- Rétention et forme de pics excellentes pour les composés polaires
- Compatibilité aqueuse 100 %
- très faible relargahe, compatible LC-MS
- Sélectivité unique
Informations légales
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Colonne de garde
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express
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What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?
1 réponse-
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What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column be stored in?
1 réponse-
Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.
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What is the Department of Transportation shipping information for this product?
1 réponse-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can I use Ascentis Express on a UHPLC system?
1 réponse-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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