If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
53829-U
Ascentis® Express 90 Å C18 (2.7 μm) HPLC Columns
L × I.D. 15 cm × 4.6 mm, HPLC Column
Synonyme(s) :
Colonne HPLC à noyau solide et couche poreuse (SPP) avec phase C18
About This Item
Produits recommandés
Nom du produit
Colonne de CLHP Ascentis® Express C18, 2,7 μm, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm
Matériaux
stainless steel column
Niveau de qualité
Agence
suitable for USP L1
Gamme de produits
Ascentis®
Caractéristiques
endcapped
Fabricant/nom de marque
Ascentis®
Conditionnement
1 ea of
Paramètres
60 °C temp. range
600 bar max. pressure (9000 psi)
Technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × D.I.
15 cm × 4.6 mm
Superficie
135 m2/g
Impuretés
<5 ppm metals
Matrice
Fused-Core particle platform
superficially porous particle
Groupe de la matrice active
C18 (octadecyl) phase
Taille des particules
2.7 μm
Dimension de pores
90 Å
pH de fonctionnement
2-9
Application(s)
food and beverages
Technique de séparation
reversed phase
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Catégories apparentées
Description générale
Rendez-vous sur la page d′accueil Ascentis Express pour obtenir plus d′informations sur cette nouvelle technologie de colonne.
Application
Analyse UHPLC/MS de l′huile de maïs sur l′Ascentis® Express C30 par comparaison à l′Ascentis®Express C18
Analyse CLHP du paracétamol et des impuretés \sur l′Ascentis®Express C18, 2,7 μm
Analyse CLHP des parabènes sur l′Ascentis®Express C18, 2,7 μm
Informations légales
Application
En option
Produit(s) apparenté(s)
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
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Chromatograms
application for HPLCapplication for HPLCapplication for HPLCapplication for HPLCAfficher plus-
How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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can this column be used at higher pH (pH 9)?
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As noted in the 'PROPERTIES' section, this column has an operating pH of 2 - 9.
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What is the part number for Phenomenex Kinetex C18 column with dimensions of 150mm (15cm) x 4.6mm ID x 2.6μm particle size?
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Unfortunately, we do not provide the Kinetex C18 column from Phenomenex. However, we offer our own alternative with similar characteristics, the Ascentis Express C18 column with a particle size of 2.7μm. The specific part number for this column is 538229-U. Please note that the minimal difference of 1μm in particle size should not significantly impact its performance.
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What are the recommended long-term storage conditions for the Ascentis® Express C18, 2.7 μm HPLC Column?
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For long-term storage of silica-based, reversed-phase columns like the Ascentis® Express C18, 2.7 μm HPLC Column, it is recommended to store them in 100% acetonitrile. For shorter periods of storage (up to 3 or 4 days), it is generally safe to store the column in most common mobile phases. However, when using buffers, it is advised to protect both the column and the HPLC equipment by flushing the column with the mobile phase that does not contain the buffer. This can be accomplished by flushing the column with a mixture of the mobile phase without the buffer (e.g., when using 60/40 ACN/buffer, flush the column with 60/40 ACN/H2O). This flushing step helps eliminate any risk of corrosion from salts and ensures quick re-equilibration of the column with the original mobile phase. Additionally, before storing the column, it is important to tightly seal the end-fittings with the provided end plugs to prevent the packing material from drying out.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Ascentis® Express HPLC Columns be used for LC-MS?
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Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
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Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
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What flow rate should I use with Ascentis® Express HPLC Columns?
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Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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Can I use Ascentis Express on a UHPLC system?
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Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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