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X4875

Xanthine Oxidase from bovine milk

Name: Xanthine Oxidase from bovine milk
Brand: SIGMA
Price: 78.1 EUR
Availability: InStock

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

Synonyme(s) :

XOD, Xanthine:oxygen oxidoreductase

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Taille/Référence	Disponibilité	Prix
10 units	Expédition prévue aujourd'huiDeSt QuentinFallavier	78,10 €
40 units	Vérifier la disponibilité des articles du panier	229,00 €
120 units	Vérifier la disponibilité des articles du panier	553,00 €

A propos de cet article

Numéro CAS:

9002-17-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-657-6

MDL number:

MFCD00082145

Numéro CE :

1.17.3.2.

Specific activity:

≥0.1 units/mg protein

Biological source:

bovine milk

78,10 €

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Propriétés

biological source

bovine milk

Quality Segment

200

type

Grade IV

form

ammonium sulfate suspension

specific activity

≥0.1 units/mg protein

storage temp.

2-8°C

Description

General description

Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers.

Formerly E.C. 1.1.3.22

Application

Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation.

Biochem/physiol Actions

Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys.

Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Physical form

Suspension in 2.3 M (NH₄)₂SO₄ containing 1 mM sodium salicylate

Analysis Note

Protein determined by biuret

Other Notes

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Comparer avec des articles similaires

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1 of 1

Cet article	X1875	X4376	X4500
Sigma-Aldrich X4875 Xanthine Oxidase from bovine milk	Sigma-Aldrich X1875 Xanthine Oxidase from bovine milk	Sigma-Aldrich X4376 Xanthine Oxidase from bovine milk	Sigma-Aldrich X4500 Xanthine Oxidase from bovine milk
description Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein	description Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein	description lyophilized powder, 0.4-1.0 units/mg protein	description Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein
specific activity ≥0.1 units/mg protein	specific activity ≥0.4 units/mg protein	specific activity 0.4-1.0 units/mg protein	specific activity ≥0.8 units/mg protein
biological source bovine milk	biological source -	biological source bovine milk	biological source bovine milk
form ammonium sulfate suspension	form ammonium sulfate suspension	form lyophilized powder	form ammonium sulfate suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200
type Grade IV	type Grade I	type -	type Grade III

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Pictogrammes

GHS08

Mot-clé

Danger

Codes de danger

H334

Mentions de précaution

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Classe de stockage

10 - Combustible liquids

Que se passe-t-il ?

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

EPI (équipement de protection individuelle)

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number

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Questions

il y a 2 années

Hello, Is there a way to find out how much 2.3M (NH4)2SO4 was used to suspend 10 Units of Xanthine Oxidase? Specifically, what is the ratio of units Xanthine Oxidase (or mg) to volume needed to create this suspension using lyophilized powder? Thank you!

1 réponse
1. Technical Support
  
  il y a 2 années
  
  The volume of a given package size varies from lot to lot. The minimum activity of this product is 0.1 units per milligram of protein. The minimum protein concentration is 5.0 mg/mL. As an example, a lot reporting an activity of 0.3 units per milligram protein at a concentration of 13.8 mg protein per milliliter, will have a volume of 2.3 mL for the 10 Unit package size. This is determined by using the following calculation:
  10U / (0.3U/mg) = 30mg / (13.8mg/ml) = 2.3 ml volume
  The lot-specific volume may be determined by data provided in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
  https://www.sigmaaldrich.com/product/sigma/x4875#product-documentation
  
  Utile ?

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