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X1875

Sigma-Aldrich

Xanthine Oxidase from bovine milk

Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein

Synonyme(s) :

XOD, Xanthine:oxygen oxidoreductase

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About This Item

Numéro CAS:
9002-17-9
Numéro de classification (Commission des enzymes):
1.17.3.2.
Numéro CE :
232-657-6
Numéro MDL:
MFCD00082145
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Type

Grade I

Forme

ammonium sulfate suspension

Activité spécifique

≥0.4 units/mg protein

Activité étrangère

uricase ≤0.5%

Conditions d'expédition

wet ice

Température de stockage

2-8°C

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Description générale

Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.
enthält auch proteolytische Aktivität

Application

Xanthine Oxidase from bovine milk has been used:
  • in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
  • in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
  • as a standard to determine XO activity
  • as a standard to test the synergistic effect of docosahexaenoic acid (DHA)

Actions biochimiques/physiologiques

Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.

Définition de l'unité

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Forme physique

Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate

Remarque sur l'analyse

Protein determined by biuret

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Xanthine oxidase inhibitors from Archidendron clypearia (Jack.) I.C. Nielsen: Results from systematic screening of Vietnamese medicinal plants
Duong NT, et al.
Asian Pacific Journal of Tropical Medicine, 10(6), 549-556 (2017)
Kurt Warnhoff et al.
Genes & development, 35(3-4), 212-217 (2021-01-16)
The molybdenum cofactor (Moco) is a 520-Da prosthetic group that is synthesized in all domains of life. In animals, four oxidases (among them sulfite oxidase) use Moco as a prosthetic group. Moco is essential in animals; humans with mutations in
M F Barroso et al.
Biosensors & bioelectronics, 26(9), 3748-3754 (2011-04-09)
In this paper, a biosensor based on a glassy carbon electrode (GCE) was used for the evaluation of the total antioxidant capacity (TAC) of flavours and flavoured waters. This biosensor was constructed by immobilising purine bases, guanine and adenine, on
Methods of Enzymatic Analysis (2012)
Antioxidant properties of propofol and erythropoietin after closed head injury in rats
Ozturk E, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 29, 922-927 (2005)
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