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Merck
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Principaux documents

SRE0027

Sigma-Aldrich

Héparine sodium salt from porcine intestinal mucosa

Synonyme(s) :

Heparin sodium salt from porcine intestinal mucosa

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.21
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

Porcine (mucosa)

Niveau de qualité

Forme

powder

Application(s)

diagnostic assay manufacturing

Chaîne SMILES 

[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O

InChI

1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-

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Autres remarques

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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

Dalia Abdelrahim Alzebdeh et al.
Journal of cellular biochemistry, 118(9), 3003-3015 (2017-03-03)
In vitro assembly of functional liver tissue is a prerequisite for the transplantation of tissue-engineered livers. There is an increasing demand for in vitro models that replicate complex events occurring in the liver. However, tissue engineering of implantable liver systems
Jae Myoung Suh et al.
Nature, 513(7518), 436-439 (2014-07-22)
Fibroblast growth factor 1 (FGF1) is an autocrine/paracrine regulator whose binding to heparan sulphate proteoglycans effectively precludes its circulation. Although FGF1 is known as a mitogenic factor, FGF1 knockout mice develop insulin resistance when stressed by a high-fat diet, suggesting a

Questions

1–2 sur 2 questions  
  1. How many times the solution of heparin can be used after resuspension in water? And what are the storage conditions of this solution?

    1 réponse
    1. There is no Product Information Sheet specific for product SE0027. There are other sodium heparin products from the porcine intestinal mucosa. The Product Information Sheet for H3393 states:
      Storage/Stability: Store the powdered product at room temperature.
      Heparin is a polysaccharide, which remains active in aqueous solution for up to 2 years at 2–8 °C, provided the solutions have been filtered through a 0.2 mm membrane. Alternatively, 0.06–1% benzyl alcohol may be added to solutions to prevent bacterial growth. Any trace of contaminating organisms in the solution will break down the heparin to use its sugars for nutrients, resulting in the degradation of non-sterile solutions.
      Heparin solutions should not be autoclaved, because additional crosslinking of the sugars may occur at high temperatures. There is no need to freeze solutions of heparin; in fact, solutions of any polysaccharide of high molecular mass should not be frozen.

      Other sources state that the pH should be between a range of pH 4 to pH 9.

      Utile ?

  2. What is the molecular weight of this product? How many milligrams? sre0027-100KU

    1 réponse
    1. The exact amount of material will vary per batch and will be based on the unit activity. For example, on the sample Certificate of Analysis, the specific activity is 206 units/mg. This means that for the 100KU pack size, there is 485 mg of material: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/SRE0027-100KUSLCQ0488.pdf

      This product does not have an indicated molecular weight, however, per Merck Index, 11th ed., p. 735, #4571: Molecular weight of heparin is 6 – 30 KDa.
      In addition, an analysis of 32 commercial samples of heparin sodium (U.S.P.) and sterile solutions of heparin sodium (U.S.P.) indicated that the average molecular weight was 9 – 12 KDa.

      Utile ?

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