As mentioned on the Product Detail Page, this product is soluble at 50 mg/mL in water. The actual activity is reported on the lot-specific Certificate of Analysis. This value should be used in determining the activity of the prepared solution. The activity specification for this product is >/=180 USP units/mg. For example, assuming an activity value of 200 units/mg, a prepared solution of 25 mg/ml will have an activity of 5000 units/ml.
H3149
Héparine sodium salt from porcine intestinal mucosa
Heparin sodium salt from porcine intestinal mucosa
Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture
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10000 UNITS
60,00 €
25000 UNITS
125,00 €
50000 UNITS
134,00 €
100000 UNITS
194,00 €
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442,00 €
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795,00 €
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Devis pour commande en gros
Sélectionner une taille de conditionnement
Changer de vue
10000 UNITS
60,00 €
25000 UNITS
125,00 €
50000 UNITS
134,00 €
100000 UNITS
194,00 €
250000 UNITS
442,00 €
500000 UNITS
795,00 €
1000000 UNITS
1 600,00 €
About This Item
60,00 €
En stockDétails
Les fabricants de produits de diagnostic devraient utiliser un produit d'une autre qualité : SRE0027
Devis pour commande en gros
Produits recommandés
Source biologique
Porcine intestinal mucosa
Niveau de qualité
Type
Grade I-A
Gamme de produits
BioReagent
Forme
powder
Activité spécifique
≥180 USP units/mg
Technique(s)
analytical sample preparation: suitable
cell culture | mammalian: suitable
Couleur
beige
Solubilité
H2O: 50 mg/mL
Application(s)
cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation
Chaîne SMILES
[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O
InChI
1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-
Clé InChI
JRTRSJGZMRQDHI-UHFFFAOYSA-N
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Description générale
L'héparine sodique, un polysaccharide sulfaté de la famille des glycosaminoglycanes, joue un rôle vital dans la recherche sur la culture cellulaire, la biologie du cancer et l'administration des médicaments. Son effet sur les interactions des protéines et des cellules en fait un composé intéressant pour l'étude des processus moléculaires et cellulaires, la modélisation des tumeurs, l'exploration des interactions de liaison et comme supplément de culture cellulaire pour assurer la viabilité des cellules.
L′héparine est principalement responsable du délai de coagulation du sang. Elle améliore l′inactivation des protéases de la voie de coagulation médiée par l′antithrombine.[1]
Application
- Le sel sodique d′héparine provenant de la muqueuse intestinale porcine a été utilisé pour la purification de sLRP (soluble circulating low density lipoprotein receptor-related protein-1) à partir de plasma humain.[2]
- Elle a été utilisée comme supplément dans le milieu M199 pour la culture des cellules endothéliales de veine ombilicale humaine.[3]
- Elle a servi d′agent anticoagulant lors de l′isolement de sang.[4]
Caractéristiques et avantages
- Composé de grande pureté convenant à une grande variété d'applications de recherche.
Autres remarques
Pour bien comprendre notre vaste gamme de polysaccharides et tout ce qu'elle peut apporter à vos recherches, nous vous encourageons à visiter notre page dédiée aux glucides.
Pour de plus amples informations sur notre gamme de Produits biochimiques, merci de compléter ce formulaire.
Produit comparable
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Certificats d'analyse (COA)
Lot/Batch Number
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
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Can I dissolve the heparin in filtered (0.22 um) nuclease free water at a concentration of 50mg/ml? Does it correspond to 5000 IU/ml? Then, I can use 15-30 IU/ ml of blood to avoid its coagulation, is it correct?
1 réponse-
Utile ?
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What is the storage condition of H3149 powder?
1 réponse-
This product should be stored in room temperature in powdered format.
Utile ?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 réponse-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Utile ?
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How can I determine the shelf life / expiration / retest date of this product?
1 réponse-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfUtile ?
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H3149-10KU heparin sodium salt is steril??
1 réponse-
The product is not sterile. Prepared solutions may be filter sterilized.
Utile ?
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please, could you explain me if this H3149 is one of 'high molecolar weight heparin'? thanks
1 réponse-
This product is a cell culture tested porcine heparin with a molecular weight range of 15-19 kDa. This material is considered a "high molecular weight heparin." According to the literaure, low molecular weight heparins are those with a molecular wieght of less that 8000 Da. See the link below to review a publication that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141002/Utile ?
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Can this material be diluted to 10,000 U/mL and stored at 2C to 8C? Do you have stability data at this temperature for lower concentrations?
1 réponse-
Yes, this product remains biologically active in dilute solutions. Stock solutions prepared at 50 mg/mL in water are stable for up to 2 years at 2 - 8°C, when filter sterilized. According to literature, heparin diluted in physiological saline at a concentration of 10 to 100 units/mL is stable for at least 35 days when stored at 2 - 8°C. Please see the link below to review the datasheet for an identical product, H3393, which may be applied to this item
H3393 Datasheet
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/608/h3393pis.pdfUtile ?
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Is the heparin sodium salt (H3149, CAS 9041-08-1) unfractionated or fractionated? How does this classification relate to its molecular weight?"
1 réponse-
The heparin sodium salt (H3149, CAS 9041-08-1) is unfractionated. This classification is reflected in its molecular weight, which ranges approximately from 9,000 to 21,000, with the majority falling between 17,000-19,000. As confirmed by our product and as indicated in the USP monograph, our heparin is unfractionated. This differs from fractionated Heparin, also known as Low Molecular Weight Heparin, which has a much lower molecular weight. According to the DBR, 3rd ed., p. 342, the molecular weight of our heparin is approximately 16,000-17,000.
Utile ?
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Can you recommend a suitable heparin product for mouse heart perfusion?
1 réponse-
Our available heparin products have not undergone specific testing for mouse heart perfusion. However, you may find H3149 of interest as it has been cited in the following publication for this intended application: "Isolation and Primary Culture of Mouse Aortic Endothelial Cells" published in J Vis Exp. 2016; (118): 52965 (PMID: 28060318).
Utile ?
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Is it a different substance from heparan sulfate?
1 réponse-
Heparin is not equivalent to heparin sulfate. Please see the link below to describe the differences:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/what-is-heparinUtile ?
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