P4653
Phosphatase, Alkaline from bovine kidney
lyophilized powder, ≥5 units/mg protein (Activity determined in diethanolamine buffer, pH 9.8)
Synonyme(s) :
Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Produits recommandés
Forme
lyophilized powder
Activité spécifique
≥5 units/mg protein (Activity determined in diethanolamine buffer, pH 9.8)
Composition
Protein, ~35% biuret
Température de stockage
−20°C
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins, such as casein, and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P4653 is from bovine kidney and is provided as a lyophilized powder containing Tris-citrate buffer salts.
Actions biochimiques/physiologiques
Alkaline phosphatase dephosphorylates proteins and nucleic acids.
Définition de l'unité
One unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. Previously sold in "glycine" units.
Forme physique
Lyophilized powder containing Tris-citrate buffer salts
Remarque sur l'analyse
Assays in Tris buffer, pH 10.2 give activity approx. 2X that in the glycine buffer system. Assays in diethanolamine buffer, pH 9.8 give activity approx. 5X that of the glycine buffer system.
Protein determined by biuret
Inhibiteur
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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M Nemir et al.
The Journal of biological chemistry, 264(30), 18202-18208 (1989-10-25)
We have reported previously that the 69-kDa major phosphoprotein, secreted by normal rat kidney (NRK) cells, is osteopontin, a glycosylated bone matrix protein. Here we show that this 69-kDa osteopontin is secreted by NRK cells in both phosphorylated (pp69) and
Lars Hansen et al.
American journal of human genetics, 92(4), 575-583 (2013-04-09)
PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)
Yuan-Kun Zhai et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(1), 105-111 (2013-04-20)
To investigate the effects of naringin on the proliferation, differention and maturaion of rat calvarial osteoblasts (ROB). Segregated neonatal SD rat skull, enzyme digestion to obtain ROB. The culture medium was replaced every three days. Serial subcultivation proceeded when cells
J A Otegbayo et al.
African journal of medicine and medical sciences, 41(4), 417-422 (2013-05-16)
HAART associated hepatoxicity is an important cause of poor adherence to therapy in HIV infected persons. An initial manifestation is elevation in the level ofAlanine Transaminase (ALT) in blood. We sought to evaluate the protective effects of Livolin, a phosphatidylcholine
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