P0497

Anti-PMP70 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-70 kDa Peroxisomal membrane Protein, Anti-ABCD3, Anti-PXMP1
• Numéro MDL: MFCD01865555
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 70 kDa
• Espèces réactives: human, mouse, rat
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Technique(s): immunoprecipitation (IP): 1-2 μg using RIPA extract (0.5 mg) from human HepG2 cells; indirect immunofluorescence: 4-8 μg/mL using mouse NIH3T3 cells; western blot (chemiluminescent): 0.5-1 μg/mL using whole extracts of rat PC12 cells or rat kidney extract or rat liver extract
• Numéro d'accès UniProt: P28288
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ABCD3(5825) ; mouse ... Abcd3(19299) ; rat ... Abcd3(25270)

Description

Description générale

The 70 kDa peroxisomal membrane protein (PMP70) is a major component of peroxisomal membranes. The peroxisome is a multifunctional single-membrane organelle associated with eukaryotic cells. PMP70 belongs to the ATP binding cassette (ABC) transporter superfamily.

Immunogène

synthetic peptide corresponding to amino acid residues 644-659 of rat PMP70 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in human.

Application

Anti-PMP70 antibody produced in rabbit has been used in:

• immunocytochemistry
• immunostaining
• immunofluorescence
• western blotting
• immunoprecipitation

Actions biochimiques/physiologiques

PMP70 participates in the metabolic transport of long and very long fatty acids into peroxisomes. PMP70 interacts and forms a stable complex with the adrenoleukodystrophy protein(ALDP), and also with several other peroxisomal proteins. ATP binding/hydrolysis by PMP70 and ALDL and their phosphorylation are involved in the regulation of fatty acid transport into peroxisomes. Mutations in the PMP70 (PXMP1) gene may cause a subset of Zellweger syndrome, an autosomal recessive disorder and is characterized by defects in import mechanism for peroxisomal matrix enzymes.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable