The kit is intended for the isolation of intact mitochondria, so most of the isolated mitochondria will contain intact inner and outer membranes. While trace amounts of mitochondria-associated membranes (MAMs) may co-purify during the process, the protocol is not optimized to enrich or preserve MAMs. Please see the Product Information Sheet at the link below for further details.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/204/819/mitoiso1pis-ms.pdf
MITOISO1
Mitochondria Isolation Kit
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
Synonyme(s) :
Mitochondria Purification Kit
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
1 KIT
883,00 €
883,00 €
Check Cart for Availability
Sélectionner une taille de conditionnement
Changer de vue
1 KIT
883,00 €
About This Item
883,00 €
Check Cart for Availability
Produits recommandés
Niveau de qualité
Utilisation
sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)
Durée de conservation
2 yr
Technique(s)
fractionation: suitable
Conditions d'expédition
dry ice
Température de stockage
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Application
Mitochondria Isolation Kit has been used:
- for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity[1]
- for the isolation of mitochondria from glomeruli[2] and heart[3]
- for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential[4]
The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.
Caractéristiques et avantages
- Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
- Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
- Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
- Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
- T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidFDS
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
10 - Combustible liquids
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
DJ-1 protects the heart against ischemia-reperfusion injury by regulating mitochondrial fission
Shimizu Y, et al.
Journal of Molecular and Cellular Cardiology, 97, 56-66 (2016)
Janna R Jackson et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 66(7), 751-764 (2011-04-02)
This study analyzed the capacity of resveratrol, a naturally occurring polyphenol, to reduce aging-induced oxidative stress and protect against sarcopenia. Middle-aged (18 months) C57/BL6 mice were randomly assigned to receive either a control diet or a diet supplemented with 0.05%
Articles
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
-
Is it possible to use the MITOISO1 kit to also isolate MAMs?
1 réponse-
Utile ?
-
-
Where is the step to get cytosol?
1 réponse-
The kit is intended for the isolation of intact mitochondria. It was not developed for cytoplasma collection. Nevertheless, reviewing the protocol, it can be assumed that the cytoplasmic fraction should be in the supernatant after the second centrifugation (point 8 of the Preparation of mitochondria from soft tissues protocol). Please keep in mind that this is not tested for suitability for cytoplasmic fraction.
Please see product MIT1000 Mitochondria/Cytosol Fractionation Kit for efficient isolation of intact mitochondria from cultured cells. This kit can be used for tissue samples as long as the tissue is first homogenized into a single cell suspension. It may be helpful to use the same protocol that is recommended for cells following homogenization of the tissue into a single cell suspension in the supplied Isotonic Mitochondrial Buffer containing protease inhibitors, then follow the kits protocol for cells: https://www.sigmaaldrich.com/product/mm/mit1000.
Utile ?
-
-
Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?
1 réponse-
The extraction buffers supplied with this kit are only available with the kit.
Utile ?
-
-
When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?
1 réponse-
Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
Utile ?
-
-
When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?
1 réponse-
If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
Utile ?
-
-
What is the Department of Transportation shipping information for this product?
1 réponse-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile ?
-
-
How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?
1 réponse-
The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin. We recommend a minimum of 50 mg.
Utile ?
-
-
Is the Product MITOISO1, Mitochondria Isolation Kit, JC-1 stain available separately?
1 réponse-
Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.
Utile ?
-
-
Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?
1 réponse-
It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose. This kit was originally designed for isolation from tissues.
Utile ?
-
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
