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CS0720

Citrate Synthase Assay Kit

1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

Synonyme(s) :

Citrate Synthase Activity Test Kit

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1 kit

Expédition prévue aujourd'huiDeSt QuentinFallavier

1 110,00 €

A propos de cet article

NACRES:
NA.28
UNSPSC Code:
12161503

1 110,00 €


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Quality Segment

usage

 kit sufficient for 100 reactions (using a 1 ml cuvette),  kit sufficient for 480 reactions (using 96 multiwell plates)

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CS(1431)

General description

The Citrate Synthase Assay Kit contains all the required reagents (including a positive control enzyme) for a fast and simple measurement of citrate synthase activity in a whole cell extract or in isolated mitochondria. In addition, the kit enables testing of the intactness of the mitochondrial inner membrane.

Biochem/physiol Actions

Citrate synthase is the initial enzyme of the tricarboxylic acid (TCA) cycle. The enzyme catalyzes the reaction of 2 carbon acetyl CoA with 4 carbon oxaloacetate to form the 6 carbon citrate. This enzyme is an exclusive marker of the mitochondrial matrix.

Analysis Note

The activity of the enzyme is measured by following the color of TNB, which is generated from DTNB present in the reaction of citrate synthesis, and caused by the deacetylation of Acetyl-CoA. The overall reaction product, TNB, absorbs at 412 nm.

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Cet article
MAK333MAK524MAK532
description

1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

description

sufficient for 100 colorimetric or fluorometric tests

description

sufficient for 100 colorimetric tests

description

sufficient for 100 colorimetric tests

Gene Information

human ... CS(1431)

Gene Information

-

Gene Information

-

Gene Information

-

Quality Level

300

Quality Level

-

Quality Level

100

Quality Level

100

usage

 kit sufficient for 480 reactions (using 96 multiwell plates),  kit sufficient for 100 reactions (using a 1 ml cuvette)

usage

sufficient for 100 colorimetric or fluorometric tests

usage

-

usage

-

shipped in

dry ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


Composants de kit seuls

Réf. du produit
Description

  • Assay buffer for citrate synthase 5x 25 mL

  • Bicine buffer 10 mL

  • CelLytic™ M Cell Lysis Reagent 10 mL

  • DTNB [5,5'-Dithiobis-(2-nitrobenzoic acid)] 4 mg

  • Acetyl coenzyme A 25 mg

  • Oxaloacetic acid 1 g

  • Citrate synthase (positive control) .1 mL

Pictogrammes

Health hazardExclamation mark

Mot-clé

Danger

Codes de danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Classe de stockage

10 - Combustible liquids

Que se passe-t-il ?

WGK 3



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Questions

1–3 sur 3 questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 réponse
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 réponse
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What are the contents of the Assay Buffer (5x) for the CS0720 citrate synthase kit?

    1 réponse
    1. The kit component B6935, the 5X assay buffer, comprises 0.5 M Tris HCl at pH 8.0.

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