CS0760
Isolated Mitochondria Staining Kit
1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)
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| Taille/Référence | Disponibilité | Prix |
|---|---|---|
1 kit | Vérifier la disponibilité des articles du panier | 513,00 € |
A propos de cet article
513,00 €
Quality Segment
usage
kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
flow cytometry: suitable, protein staining: suitable
fluorescence
λex 490 nm; λem 590 nm (JC-1 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
dry ice
storage temp.
−20°C
Application
Biochem/physiol Actions
Features and Benefits
- Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
- Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation
Analysis Note
1 of 1
Cet article | |||
|---|---|---|---|
| description 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates) | description 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions) | description sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues | description sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells |
| detection method fluorometric | detection method fluorometric | detection method - | detection method - |
| usage kit sufficient for 50 reactions (in a 2 mL cuvette), kit sufficient for 1000 reactions (using 96 multiwell plates) | usage kit sufficient for 40 tests (of 5 mL cell suspensions), kit sufficient for 200 tests (of 1 mL cell suspensions) | usage sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL) | usage sufficient for 50 applications (2-5 x 107 cells) |
| technique(s) protein staining: suitable, flow cytometry: suitable | technique(s) protein staining: suitable, flow cytometry: suitable | technique(s) fractionation: suitable | technique(s) ELISA: suitable, western blot: suitable |
| fluorescence λex 490 nm; λem 590 nm (JC-1 dye) | fluorescence λex 490 nm; λem 530 nm (green) (JC-1 monomers), λex 525 nm; λem 590 nm (red) (JC-1 aggregates) | fluorescence - | fluorescence - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage condition dry at room temperature | storage condition dry at room temperature | storage condition - | storage condition - |
Composants de kit seuls
- DMSO 1 mL
- JC-1 Assay Buffer 5X 25 mL
- JC-1 Stain 25 μg
- Valinomycin Ready Made 100 μL
Mot-clé
Danger
Codes de danger
Mentions de précaution
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Oral
Classe de stockage
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Que se passe-t-il ?
WGK 3
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