MAK186

Xanthine/Hypoxanthine Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

• Synonyme(s) : Xanthine and Hypoxanthine Detection Kit
• Numéro CE : 200-718-6
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Méthode de détection: colorimetric; fluorometric
• Maladie(s) pertinente(s): neurological disorders; rheumatological diseases
• Température de stockage: −20°C

Description

Description générale

Xanthine is a purine base present in body fluids, tissues, and plants cells. It is also found in urinary calculi. Xanthine is formed by the oxidation of hypoxanthine during the degradation of adenosine monophosphate (AMP) to uric acid. Xanthine/ hypoxanthine measurements in plasma and urine have been used to monitor allopurinol therapy. Cerebrospinal fluid (CSF) xanthine/hypoxanthine levels have been used as therapeutic guides and disease progression markers in hydrocephalus.

The Xanthine/Hypoxanthine Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the xanthine/hypoxanthine present.
Note: The fluorometric assay is ~10 times more sensitive than the colorimetric assay.

Caractéristiques et avantages

Compatible with high-throughput liquid handling systems.

Adéquation

Suitable for the detection of Xanthine/Hypoxanthine in a variety of samples such as tissue, cells, and body fluids.

Principe

The Xanthine/Hypoxanthine Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the xanthine/hypoxanthine present.

Autres remarques

The fluorometric assay is ~10 times more sensitive than the colorimetric assay.

Composants de kit seuls

• Xanthine Assay Buffer
• Peroxidase Substrate
• Xanthine Enzyme Mix
• Developer
• Xanthine Standard

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334,H412
• Conseils de prudence: P261 - P272 - P273 - P280 - P284 - P302 + P352
• Classification des risques: Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids