The cell pellet can be stored at -80°C with a small amount of PBS, avoiding storage with culture medium. Upon thawing, it's recommended to wash the cells once with PBS and then remove the PBS. Following this step, the assay buffer should be used for extraction according to the kit instructions. It's important to avoid multiple freeze-thaw cycles. However, the supplier does not specify the maximum time frozen cells will remain stable for assay.
MAK078
Xanthine Oxidase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonyme(s) :
XO Activity Assay Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 100 colorimetric or fluorometric tests
Méthode de détection
colorimetric
fluorometric
Maladie(s) pertinente(s)
rheumatological diseases; gastrointestinal diseases; cardiovascular diseases
Température de stockage
−20°C
Informations sur le gène
human ... XDH(7498)
mouse ... XDH(22436)
rat ... XDH(497811)
Description générale
Xanthine Oxidase (XO) catalyzes the sequential oxidation of hypoxanthine to xanthine, and xanthine to uric acid and hydrogen peroxide. In humans and other primates, XO controls the final step of purine catabolism and is normally found in the liver and the intestinal mucosa. In rodents, XO is broadly expressed in most tissues. While XO activity is normally very low in blood, liver injury can result in the release of XO into blood. XO may contribute to the pathogenesis of gout and cardiovascular disease, and XO activity or expression may be upregulated in these conditions.
Application
Adéquation
Suitable for the measurement of xanthine oxidase in a variety of samples including tissue, cells and serum
Principe
The Xanthine Oxidase Activity Assay Kit provides a simple and direct procedure for measuring XO activity in a variety of biological samples. XO activity is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the hydrogen peroxide generated. One unit of XO is defined as the amount of enzyme that catalyzes the oxidation of xanthine, yielding 1.0 μmole of uric acid and hydrogen peroxide per minute at 25 °C.
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Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
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Les clients ont également consulté
Neelam Malik et al.
BMC chemistry, 13(1), 53-53 (2019-08-07)
Hesperitin, a naturally occurring flavonoid was hybridized with phenolic acids to evaluate its potential to inhibit the activity of xanthine oxidase (XO), a key enzyme which catalyses xanthine to uric acid which is found to be associated with gout and
Neelam Malik et al.
Medicinal chemistry (Shariqah (United Arab Emirates)), 16(5), 643-653 (2019-10-05)
Xanthine oxidase (XO; EC 1.17.3.2) has been considered as a potent drug target for the cure and management of pathological conditions prevailing due to high levels of uric acid in the bloodstream. The role of xanthine oxidase has been well
Probiotic therapy alleviates hyperuricemia in C57BL/6 mouse model.
Cao T, et al.
Biomedical Research, 28(5) (2017)
Shivangi Rastogi et al.
PLoS pathogens, 17(7), e1009712-e1009712 (2021-07-30)
Mycobacterium tuberculosis (Mtb) has evolved to evade host innate immunity by interfering with macrophage functions. Interleukin-1β (IL-1β) is secreted by macrophages after the activation of the inflammasome complex and is crucial for host defense against Mtb infections. We have previously
Ha Thi Nguyen et al.
PloS one, 16(6), e0253572-e0253572 (2021-07-01)
In the present study, in silico predictions and molecular docking were performed on five clerodane diterpenes (1-5) from Polyalthia longifolia seeds to evaluate their potential as xanthine oxidase (XO) inhibitors. The initial screening was conducted by target prediction using TargetNet
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Does the kit allow the use of cell pellets that have been frozen at -80°C, or do the samples need to be fresh, considering that the cells should be rapidly homogenized with the assay buffer?
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