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MAK074

Sigma-Aldrich

Ascorbic Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Vitamin C Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Méthode de détection

colorimetric
fluorometric

Température de stockage

−20°C

Description générale

The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Adéquation

Suitable for use with biological fluids, food samples, and growth media. Proteins present in the sample can interfere with this assay. Protein containing samples are best analyzed using the Ascorbic Acid Assay II (MAK075). Alternatively, samples can be deproteinized using a 10 kDa Molecular Weight Cut-Off Spin Filter.

Principe

In this assay, Ascorbic Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/ λem = 587 nm) product, proportional to the ascorbic acid present.

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Réf. du produit
Description
Tarif

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H319,H334

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8L15K06610
7F22K06610
6B18K06610
6G20K06610
6A30K06610

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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Ermenegilda Vitale et al.
Plants (Basel, Switzerland), 10(5) (2021-05-01)
This paper evaluates the combined effect of biostimulant and light quality on bioactive compound production and seedling growth of soybean (Glycine max L. Merrill) plants. Germinated seeds pre-treated with different concentrations (0.01%, 0.05%, 0.5%) of an amino acid-based biostimulant were
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Antioxidants (Basel, Switzerland), 11(2) (2022-02-26)
In this work, we assess the potential of waste products of Phlegrean mandarin (Citrus reticulata Blanco), namely seeds and peel, to be reutilized as a source of bioactive compounds beneficial for the human diet. Starting from the evidence that the
Giulia Costanzo et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-06-18)
Peel, pulp and seed extracts of three mandarin varieties, namely Phlegraean mandarin (Citrus reticulata), Kumquat (Citrus japonica), and Clementine (Citrus clementina) were compared and characterised in terms of photosynthetic pigment content, total polyphenols amount, antioxidant activity and vitamin C to
Manas R Gartia et al.
Scientific reports, 5, 16011-16011 (2015-11-04)
Limited training, high cost, and low equipment mobility leads to inaccuracies in decision making and is concerning with serious ocular injuries such as suspected ruptured globe or post-operative infections. Here, we present a novel point-of-service (POS) quantitative ascorbic acid (AA)
Stephanie P DiTroia et al.
Nature, 573(7773), 271-275 (2019-09-06)
Development is often assumed to be hardwired in the genome, but several lines of evidence indicate that it is susceptible to environmental modulation with potential long-term consequences, including in mammals1,2. The embryonic germline is of particular interest because of the
Afficher tous les articles scientifiques apparentés

Questions

1–2 sur 2 questions  

  1. What is the limit of detection for this assay?

    1 réponse

    1. This assay is designed for the detection of ascorbic acid and is capable of detecting 0.01-10 nmol of ascorbic acid per assay.

      Utile ?

  2. What is the minimum detectable limit for your Ascorbic acid color metric assay kit. What is the accuracy level +/- ppm.

    1 réponse

    1. While the absolute minimal limit has not been determined, this kit is intended and the standard curve is set up to detect 0.01-10 nmol of ascorbic acid per assay.

      Please see the link below to the product datasheet for further information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/590/655/mak074bul.pdf

      Utile ?

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