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MAK039

Sigma-Aldrich

Acetyl-Coenzyme A Assay Kit

sufficient for 100 fluorometric tests

Synonyme(s) :

Acetyl-CoA Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 fluorometric tests

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

cancer; neurological disorders

Température de stockage

−20°C

Description générale

Acetyl-CoA is an essential cofactor and carrier of acyl groups in enzymatic acetyl transfer reactions. It is formed either by the oxidative decarboxylation of pyruvate in mitochondria, by the oxidation of long-chain fatty acids, or by the oxidative degradation of certain amino acids. Acetyl-CoA is the starting compound for the citric acid cycle (Kreb′s cycle). It is also a key precursor in lipid biosynthesis, and the source of all fatty acid carbons. Acetyl-CoA positively regulates the activity of pyruvate carboxylase. It is a precursor of the neurotransmitter acetylcholine. Histone acetylases (HAT) use Acetyl-CoA as the donor for the acetyl group used in the post-translational acetylation reactions of histone and non-histone proteins.

Acetyl-Coenzyme A acts as an indicator of fat, sugar, and protein levels and shows up on the nutritional status. Thus, starvation greatly reduces the level of acetyl-CoA, which stimulates the process of autophagy. In lowered glucose condition, acetyl-CoA participates in the production of ATP. Increased acetyl-CoA levels are observed in prostate cancer due to elevated fatty acid utilization and thus provides an additional energy source for the tumor cell growth. Nutrition deprivation, changes the levels of certain metabolites, including acetyl-CoA, which stimulates epigenetic modifications that would affect the central nervous system.

Application

Acetyl-Coenzyme A Assay Kit has been used to quantify the level of acetyl-CoA in astrocytes exposed to recombinant HIV protein Tat and Cocaine. It has also been used to study the mitochondrial metabolism by evaluating the levels of acetyl-CoA using hypoxia induced stress in primary human ventricular cardiomyocytes.

Adéquation

Suitable for the measurement of Acetyl CoA in a variety of biological samples

Principe

Acetyl-CoA concentration is determined by a coupled enzyme assay, which results in a fluorometric (λex = 535/λem = 587 nm) product, proportional to the Acetyl-CoA present. Typical sensitivities of detection for this kit are 10-1000 pmole of Acetyl CoA. This kit is a highly sensitive assay for determining Acetyl-CoA level in a variety of biological samples.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Maria I Dauden et al.
Science advances, 5(7), eaaw2326-eaaw2326 (2019-07-17)
The highly conserved Elongator complex modifies transfer RNAs (tRNAs) in their wobble base position, thereby regulating protein synthesis and ensuring proteome stability. The precise mechanisms of tRNA recognition and its modification reaction remain elusive. Here, we show cryo-electron microscopy structures
Nuclear-cytoplasmic localization of acetyl coenzyme a synthetase-1 in the rat brain
Ariyannur P S, et al.
The Journal of Comparative Neurology, 518(15), 2952-2977 (2010)
Lilong He et al.
Plant physiology, 180(1), 198-211 (2019-02-17)
Cadmium (Cd) is a major heavy metal pollutant, and Cd toxicity is a serious cause of abiotic stress in the environment. Plants protect themselves against Cd stress through a variety of pathways. In a recent study, we found that mitochondrial
Fatty acid oxidation is a dominant bioenergetic pathway in prostate cancer
Liu Y.
Prostate Cancer and Prostatic Diseases, 9(3), 230-230 (2006)
Nanocurcumin prevents hypoxia induced stress in primary human ventricular cardiomyocytes by maintaining mitochondrial homeostasis.
Nehra S, et al.
PLoS ONE, 10(9), e0139121-e0139121 (2015)
Afficher tous les articles scientifiques apparentés

Articles

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Aerobic Glycolysis and the Warburg Effect

Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.

Fatty Acid Synthesis in Cancer Cells

Fatty acid synthesis supports cancer cell proliferation, essential for membrane generation, protein modification, and bioenergetics.

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