MAK113
ATPase/GTPase Activity Assay Kit
sufficient for 200 colorimetric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Utilisation
sufficient for 200 colorimetric tests
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
cancer
Température de stockage
2-8°C
Catégories apparentées
Description générale
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.
Principe
The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Met. Corr. 1
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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PloS one, 12(7), e0180241-e0180241 (2017-07-26)
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Nature communications, 8(1), 1518-1518 (2017-11-16)
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International journal of biological sciences, 15(12), 2522-2537 (2019-11-23)
Despite remarkable advancements in our understanding of breast cancer, it remains the leading cause of cancer deaths in women. Distant recurrence and metastasis is the main reason for death due to breast cancer. It is well recognized that the GATA
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