This lectin is soluble in phosphate buffered saline at pH 7.2 (1 mg/ml). Solutions may be stored frozen and protected from light in aliquots at –20 °C. Prolonged storage and repeated freezing and thawing are not recommended.
L7381
Lectin from Arachis hypogaea (peanut)
FITC conjugate, lyophilized powder
Synonyme(s) :
PNA, Peanut agglutinin
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
1 MG
88,30 €
2 MG
152,00 €
5 MG
337,00 €
88,30 €
Disponible pour expédition le07 avril 2025Détails
Sélectionner une taille de conditionnement
Changer de vue
1 MG
88,30 €
2 MG
152,00 €
5 MG
337,00 €
About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
88,30 €
Disponible pour expédition le07 avril 2025Détails
Produits recommandés
Conjugué
FITC conjugate
Niveau de qualité
Forme
lyophilized powder
Composition
Protein, ~10% Lowry
Ampleur du marquage
4-8 mol FITC per mol protein
Température de stockage
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Catégories apparentées
Description générale
Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.[1]
Application
Lectin from Arachis hypogaea (peanut) has been used:
- to determine the acrosomal status (presence or absence of acrosomal matrix corresponding to intact or acrosome-reacted spermatozoa) on viable sperm cells[2]
- to probe cryosections of mediastinal lymph nodes (mLNs) with fluorochrome-labeled PNA and anti-B220 to detect germinal centers (GCs)[3]
- to visualize the cone outer segments of pre-treated retinal sections[4]
Actions biochimiques/physiologiques
Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.[1]
PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase treated erythrocytes. PNA has potent anti-T activity similar to the anti-T antibody in human sera. The lectin can be used to distinguish between human lymphocyte subsets.
Forme physique
Contains phosphate buffer salts and NaCl
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Caner Öztürk et al.
Acta veterinaria Hungarica, 65(3), 429-439 (2017-09-29)
The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and
NeuroD1 regulates expression of thyroid hormone receptor beta2 and cone opsins in the developing mouse retina
Liu H, et al.
The Journal of Neuroscience, 28(3), 749-756 (2008)
Napapach Chuaychu-Noo et al.
Cryobiology, 74, 8-12 (2017-01-04)
Little is known about the effects of Cholesterol-Loaded Cyclodextrin (CLC) on post-thaw semen quality in chicken. The aim of the present study is to investigate the efficacy of CLC levels (0, 1, 2 and 3 mg/mL Schramm diluent) on post-thawed semen
Interleukin-2 inhibits germinal center formation by limiting T follicular helper cell differentiation
Ballesteros Tato A, et al.
Immunity, 36(5), 847-856 (2012)
Centimorgan-range one-step mapping of fertility traits using interspecific recombinant congenic mice
Lhote D, et al.
Genetics, 176(3), 1907-1921 (2007)
-
The product information document specifies -20C storage of the lyophilized product. Once resuspended in PBS, should it be stored at 4C instead?
1 réponse-
Utile ?
-
-
If samples are stored in 100% ethanol, it there an issue for the actual binding of the lectin to surface sugars?
1 réponse-
Fixation generally influences subsequent staining procedures. A recommended approach would be to fix identical tissue sections in alcohol and 10% Neutral Buffered Formalin and then determine which method yields the best staining outcome.
Utile ?
-
-
Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein? If so, what is the carbohydrate content?
1 réponse-
Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate. Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure". Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).
Utile ?
-
-
What is the Department of Transportation shipping information for this product?
1 réponse-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile ?
-
-
How does the storage temperature relate to shipping conditions?
1 réponse-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
Utile ?
-
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
