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L5667

Sigma-Aldrich

LB Agar Ampicillin-100, Plates

pre-poured LB agar plates with 100 μg/ml ampicillin

Synonyme(s) :

ampicillin agar plates

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About This Item

Code UNSPSC :
41121800

Qualité

for molecular biology

Stérilité

sterile; autoclaved

Forme

plates (10 cm)
plates (10cm, 20-25ml each)

Disponibilité

available only in USA

Capacité

20-25 mL

Température de stockage

2-8°C

Adéquation

selective for Escherichia coli
selective for coliforms

Description générale

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

LB Agar Ampicillin-100, Plates has been used to plate and incubate the mixture of thawed bacteria and plasmid solution for vimentin expression. It has also been used in the generation of the Trypanosoma brucei RNAi library.

Caractéristiques et avantages

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

Composants

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
100μg/ml ampicillin

Autres remarques

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Linfeng Xu et al.
Nature communications, 12(1), 6803-6803 (2021-11-25)
Enzymes are represented across a vast space of protein sequences and structural forms and have activities that far exceed the best chemical catalysts; however, engineering them to have novel or enhanced activity is limited by technologies for sensing product formation.
Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes
Glover L, et al.
Nature Protocols, 10(1), 106-106 (2015)
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science Advances, 4(6) (2018)
Viscoelastic properties of vimentin originate from nonequilibrium conformational changes
Block J, et al.
Science advances, 4(6) (2018)
Daniel Del Valle Morales et al.
Bio-protocol, 10(20), e3791-e3791 (2021-03-05)
The 5' cap is a ubiquitous feature of eukaryotic mRNAs. It is added in the nucleus onto newly synthesized pre-mRNA, and in the cytoplasm onto mRNAs after decapping or endonuclease cleavage. Cytoplasmic recapping can occur after loss of the cap

Articles

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

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