L0382

Lipase from porcine pancreas

Type VI-S, ≥20,000 units/mg protein, lyophilized powder

• Synonyme(s) : PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numéro CAS: 9001-62-1
• Numéro de classification (Commission des enzymes): 3.1.1.3 (BRENDA, IUBMB)
• Numéro CE : 232-619-9
• Numéro MDL: MFCD00131509
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Type: Type VI-S
• Niveau de qualité: 300
• Forme: lyophilized powder
• Activité spécifique: ≥20,000 units/mg protein
• Composition: Protein, 40-70%
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Clé InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

Description générale

Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwich domain.[1] PPL belongs to a subclass of the carboxylesterases family.[2]

Application

Lipase from porcine pancreas has been used:

• in lipase inhibition assay with plant extracts[3]
• in in vitro digestion gelatinized starch/fiber mixtures[4]
• with simulated intestinal fluid to mimic the digestive tract condition of by L. fermentum PC1 in fermented oats[5]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Actions biochimiques/physiologiques

Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms.[2] PPL is cost-effective and is useful in biotransformation reactions.[1] It displays high selectivity, and catalytic activity.[1] PPL is also used in the detergent formulation for removing oil stains from clothes.[1]

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Définition de l'unité

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)