62305

Lipase from Rhizopus oryzae

powder (fine), ~10 U/mg

• Synonyme(s) : Lipase from Rhizopus arrhizus, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numéro CAS: 9001-62-1
• Numéro de classification (Commission des enzymes): 3.1.1.3 (BRENDA, IUBMB)
• Numéro CE : 232-619-9
• Numéro MDL: MFCD00131509
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: powder (fine)
• Niveau de qualité: 100
• Activité spécifique: ~10 U/mg
• Poids mol.: M_r ~43000
• Température de stockage: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Clé InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

Description générale

Lipase from Rhizopus oryzae (ROL) comprises an oxyanion hole, four N-glycosylation sites, and an active site region. It possesses N-terminal presequence and prosequence.

Research Area: Cell Signaling
Rhizopus oryzae lipase (ROL) is a protein synthesized in a precursor form that includes a presequence of 26 amino acids, followed by a prosequence of 97 amino acids, which is attached to the N-terminal of a mature sequence consisting of 269 amino acids.

Application

Lipase from Rhizopus oryzae has been used:

• to test its effect on 1,2-diolein synthesis and triolein ethanolysis
• for immobilization on graphene oxide support for biocatalysis studies
• to digest triglycerides (TAG) from Chlamydomonas reinhardtii and S. cerevisiae

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Actions biochimiques/physiologiques

Rhizopus oryzae lipase (ROL) has been extensively researched for its regiospecificity in biodiesel production. Due to its remarkable characteristics, including 1,3-specificity, high enantioselectivity, and stability in organic solvents, ROL has garnered significant attention for applications in the energy, food, and pharmaceutical industries.

Lipase from Rhizopus oryzae (ROL) acts as a catalyst for the enzymatic biosynthesis of polyglycerol polyricinoleate through a reversal of hydrolysis. ROL is useful in the industrial production of structured lipids due to its 1,3-regiospecificity functionality.

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Définition de l'unité

1 U corresponds to the amount of enzyme which liberates 1 μmol of butyric acid per minute at pH 8.0 and 40°C (tributyrin, Cat. No. 91010 as substrate) 5000 U as described above are equivalent to ~1 U using triolein, Cat. No. 62314 as substrate, at pH 8.0 and 40°C

Autres remarques

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Catalyst for the interesterification of oils and fats; For removal of interfering triglycerides in the electroimmunoassay of apolipoprotein B; Racemic epoxy ester resolution through enantioselective enzymatic hydrolysis

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)