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F7378

Sigma-Aldrich

Fluorescein diacetate

Powder

Synonyme(s) :

3,6-Diacetoxyfluoran, Di-O-acetylfluorescein

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About This Item

Formule empirique (notation de Hill):
C24H16O7
Numéro CAS:
596-09-8
Poids moléculaire :
416.38
Beilstein:
62575
Numéro CE :
209-877-6
Numéro MDL:
MFCD00005062
Code UNSPSC :
12171500
ID de substance PubChem :
24894939
Nomenclature NACRES :
NA.47
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Nom du produit

Fluorescein diacetate, used as cell viability stain

Niveau de qualité

200

Forme

powder

Technique(s)

titration: suitable

Couleur

white to faint yellow

Pf

200-203 °C (lit.)

Solubilité

acetone: 25 mg/mL

ε (coefficient d'extinction)

≥86000 at 488-494 nm in 0.1 M NaOH

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3

Clé InChI

CHADEQDQBURGHL-UHFFFAOYSA-N

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Catégories apparentées

Description générale

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA.[1]

Application

Fluorescein diacetate has been used for the differentiation of live cells from dead cells.[2][3]

Substrats

Lipase substrate

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

06693

Timestrip Plus™ -20 °C

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000413679
0000413679
0000344082
0000344082
MKCM9758

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

The influence of scaffold microstructure on chondrogenic differentiation of mesenchymal stem cells.
Zhang J
Biomedical Materials (Bristol, England), 9, 035011-035011 (2014)
Effect of shock waves on suspended and immobilized L1210 cells.
Brummer F
Ultrasound in Medicine & Biology, 15, 229-229 (1989)
Cell Growth, Differentiation and Senescence: A Practical Approach (1999)
Melina M Malinen et al.
Tissue engineering. Part A, 18(23-24), 2418-2425 (2012-06-21)
Current hepatocyte models do not mimic the human liver morphology and functions properly and, therefore, drug metabolism, excretion, and toxicity in the liver are inadequately predicted. In this study, we established three-dimensional (3D) hepatic cell cultures in hydrogels of peptide
Derek Toms et al.
Frontiers in bioengineering and biotechnology, 8, 534-534 (2020-06-26)
We have developed an accessible software tool (receptoR) to predict potentially active signaling pathways in one or more cell type(s) of interest from publicly available transcriptome data. As proof-of-concept, we applied it to mouse photoreceptors, yielding the previously untested hypothesis
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Questions

1–6 sur 6 questions  

  1. What are some common applications for Product F7378, Fluorescein diacetate?

    1 réponse

    1. This compound is used extensively for the staining of living cells.  It has been used as a viability stain for cultured neurons and plant ways, and in measuring of esterase and cholinesterase activity. See the following references for usage: Fluorometric determination of lipase: Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes", Anal. Chem. 36, 409, 1964. ; Anal. Biochem., Vol. 14, 28 (1966);Fluorescent stain for viable rosette-forming cells: J. Immun. Meth., Vol. 5,305 (1974);Fluorogenic substrate detection of viable pathogenic protozoa: Science, Vol. 227, 435 (1985);Reference Standard in antibody studies: Anal. Biochem., Vol. 9, 474 (1964);Cell viability staining: Staining Procedures, G. Clark, 4th ed., p. 93.Guilbault, G.G. and Kramer, D.N., "Fluorometric Determination of Lipase, Acylase, Alpha- and Gamma- Chymotrypsin and Inhibitors of These Enzymes, Anal. Chem. 36, 409, 1964.""   "

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  2. After Product F7378, Fluorescein diacetate, has been converted to fluorescein, what are the maximum excitation and emission wavelengths?

    1 réponse

    1. The maximum excitation wavelength is 490 nm and the maximum emission wavelength is 526 nm.

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  3. How stable are solutions of Product F7378, Fluorescein diacetate, once prepared?

    1 réponse

    1. Based on our experience, stock solutions of fluorescein diacetate at 5 mg/mL in acetone or DMSO can be stored at -20 °C. According to one on-line source, solutions at 5 mg/mL in acetone or ethanol can be stored refrigerated.

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  4. Is Product F7378, Fluorescein diacetate, a fluorescent compound?

    1 réponse

    1. No. Only upon hydrolysis of the esters, catalyzed by cellular esterase, the lactone ring opens yielding fluorescein.  Only the fluorescein form is fluorescent.

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  5. What is the Department of Transportation shipping information for this product?

    1 réponse

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. What solubility information do you have on Product F7378, Fluorescein diacetate?

    1 réponse

    1. We have found that it is slightly soluble in water at 0.1%, and in ethanol at 0.3%. It is also soluble in dimethylformamide (DMF).

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