81845

Iodure de propidium

≥94% (HPLC)

• Synonyme(s) : Diiodure de 3,8-diamino-5-[3-(diéthylméthylammonio)propyl]-6-phénylphénanthridinium diiodide
• Formule empirique (notation de Hill): C₂₇H₃₄I₂N₄
• Numéro CAS: 25535-16-4
• Poids moléculaire : 668.39
• Beilstein: 3843838
• Numéro CE : 247-081-0
• Numéro MDL: MFCD00011921
• Code UNSPSC : 12352200
• ID de substance PubChem : 329769221
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 200
• Essai: ≥94% (HPLC)
• Forme: powder
• Pf: 220-225 °C (dec.) (lit.)
• Fluorescence: λ_ex 482 nm; λ_em 608 nm (pH 7.2); λ_ex 540 nm; λ_em 608 nm (bound to DNA)
• Température de stockage: 2-8°C
• Chaîne SMILES: [I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
• InChI: 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• Clé InChI: XJMOSONTPMZWPB-UHFFFAOYSA-M

Description

Description générale

PI is used for selective labelling of dead cells.[1]

Propidium iodide is a fluorescent intercalating agent used for staining.[2] It functions as an apoptosis marker[3] and has linear DNA-binding capacity.[4] Propidium iodide is an effective indicator of tissue injury.[5]

Application

Propidium iodide has been used:

• to stain DNA in cell cycle analysis[6]
• to stain cells[7] and cell nuclei[8][9]
• for the preparation of propidium iodide (PI)stock solution[10]
• to stain cells for flow cytometry[11][12]

in scrape-loading dye transfer assay[13]

Caractéristiques et avantages

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Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Warning
• Mentions de danger: H341
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Muta. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves