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B3111

Sigma-Aldrich

ANTI-FLAG® M2 antibody, Mouse monoclonal

Clone M2, purified from hybridoma cell culture in bioreactor

Synonyme(s) :

Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG

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0.2 MG
493,00 €
1 MG
965,00 €

493,00 €

Date d'expédition estimée le14 avril 2025

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0.2 MG
493,00 €
1 MG
965,00 €

About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

493,00 €

Date d'expédition estimée le14 avril 2025

Source biologique

mouse

Forme d'anticorps

purified immunoglobulin (purified IgG1 subclass)

Clone

M2, monoclonal

Durée de conservation

4 yr

Produit purifié par

using Protein A

Température de stockage

−20°C

Catégories apparentées

Description générale

Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.

form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL

Application

IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.

Learn more product details in our FLAG® application portal.

Conditionnement

polypropylene screw cap vial

Notes préparatoires

Dilute the antibody solution from 0.5-10 ug/mL in specified buffer

Informations légales

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000420002
0000420002
0000395436
0000388873
0000388873

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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Runming Zeng et al.
Experimental biology and medicine (Maywood, N.J.), 246(6), 644-653 (2020-12-11)
Osteoarthritis (OA), the most prevalent form of arthritis disease, is characterized by destruction of articular cartilage, osteophyte development, and sclerosis of subchondral bone. Transcription factors Janus kinase 1/signal transducer and activator of transcription 3 (JAK1/STAT3) and Forkhead box M1 (FOXM1)
Rui Santalla Méndez et al.
Cellular and molecular life sciences : CMLS, 80(10), 306-306 (2023-09-27)
Intracellular vesicle transport is essential for cellular homeostasis and is partially mediated by SNARE proteins. Endosomal trafficking to the plasma membrane ensures cytokine secretion in dendritic cells (DCs) and the initiation of immune responses. Despite its critical importance, the specific
FBXO42 facilitates Notch signaling activation and global chromatin relaxation by promoting K63-linked polyubiquitination of RBPJ.
Jiang, et al.
Science Advances, 8, eabq4831-eabq4831 (2022)
Arturo Roca-Rivada et al.
JCI insight, 9(9) (2024-05-08)
The importance of the proper localization of most receptors at the cell surface is often underestimated, although this feature is essential for optimal receptor response. Endospanin 1 (Endo1) (also known as OBRGRP or LEPROT) is a protein generated from the
Weixiang Bian et al.
STAR protocols, 3(3), 101569-101569 (2022-07-26)
Identification of protein interactors is fundamental to understanding their functions. Here, we describe a modified protocol for tandem affinity purification coupled with mass spectrometry (TAP/MS), which includes two-step purification. We detail the S-, 2×FLAG-, and Streptavidin-Binding Peptide (SBP)- tandem tags
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