The M2 Flag antibody is linked to the resin through a covalent linkage to one heavy chain. However, during the conjugation process, some Flag antibodies may also become bound to the resin through noncovalent attachments. To mitigate this, it is recommended to wash the resin using 0.1M Glycine followed by equilibration before use. This washing step ensures that the noncovalently linked antibodies are washed off. The protocol for this product can be found on the product page under more documents, where the Product Information sheet is available.
M8823
Billes magnétiques Anti-FLAG® M2
Anti-FLAG® M2 Magnetic Beads
1 sur 1 consommateurs ont reçu un échantillon de produit ou ont participé à une promotion
affinity isolated antibody
Synonyme(s) :
Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse, Résine FLAG® pour analyse à haut débit, Résine d'affinité Flag®, Résine d'affinité magnétique FLAG®, Anti-ddddk, Anti-dykddddk
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Conjugué
magnetic beads
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
M2, monoclonal
Forme
suspension
Durée de conservation
2 yr at -20 °C
Classe(s) chimique(s) de l'analyte
proteins
Technique(s)
affinity chromatography: suitable
immunoprecipitation (IP): suitable
Taille des billes
20-75 μm
Matrice
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
Isotype
IgG1
Capacité
≥0.6 mg/mL binding capacity
Conditions d'expédition
wet ice
Température de stockage
−20°C
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Description générale
Les billes magnétiques Anti-FLAG M2 sont des billes d'agarose à 4 % recouvertes d'anticorps (monoclonal de souris) Anti-FLAG M2. L'anticorps M2 reconnaît la séquence FLAG de l'extrémité N-terminale, de l'extrémité Met-N-terminale et de l'extrémité C-terminale. Cela permet de détecter et de capturer les protéines de fusion contenant une séquence peptidique FLAG.
Application
Compatible avec les techniques d'immunoprécipitation.
Élution - peptide FLAG®, glycine, pH 3,5, peptide 3x FLAG®.
Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.
Élution - peptide FLAG®, glycine, pH 3,5, peptide 3x FLAG®.
Pour en savoir plus sur nos produits, rendez-vous sur notre portail des applications des produits FLAG®.
Caractéristiques et avantages
Les propriétés magnétiques permettent de :
- Réaliser des séparations très rapides
- Accélérer de manière significative les manipulations, par exemple les lavages répétitifs
- Traiter de nombreux échantillons à l'aide de plaques
Ce qui vous permet d'obtenir :
- Des expérimentations plus rapides
- Une meilleure reproductibilité
- Une quantification plus précise des protéines étudiées
- Réaliser des séparations très rapides
- Accélérer de manière significative les manipulations, par exemple les lavages répétitifs
- Traiter de nombreux échantillons à l'aide de plaques
Ce qui vous permet d'obtenir :
- Des expérimentations plus rapides
- Une meilleure reproductibilité
- Une quantification plus précise des protéines étudiées
Forme physique
Produit fourni sous forme de suspension à 50 % dans du glycérol à 50 % avec du phosphate de sodium 10 mM, du chlorure de sodium 150 mM (pH 7,4) et 0,02 % (p/v) d'azide de sodium (PBA/A).
Autres remarques
Informations légales
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Faites votre choix parmi les versions les plus récentes :
Certificats d'analyse (COA)
Lot/Batch Number
Vous ne trouvez pas la bonne version ?
Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Sachin Jain et al.
Frontiers in microbiology, 9, 1316-1316 (2018-07-03)
The Bcl-2 associated athanogene (Bag) family is a multifunctional group of proteins distinguished by a conserved region known as the Bag domain (BD). Herein, we discuss the discovery and characterization of a Bag protein in the model genetic fungus Aspergillus
Chanqiong Zhang et al.
Cancer biology & therapy, 20(9), 1213-1222 (2019-04-16)
It is verified that long non-coding RNAs (lncRNAs) play crucial roles in various cancers. LncRNA LEF1-AS1 is a reported oncogene in colorectal cancer and glioblastoma. In this study, we unveiled that LEF1-AS1 markedly increased in oral squamous cell carcinoma (OSCC)
K Kollmann et al.
Leukemia, 31(4), 934-944 (2016-10-16)
Most myeloproliferative neoplasm (MPN) patients lacking JAK2 mutations harbour somatic CALR mutations that are thought to activate cytokine signalling although the mechanism is unclear. To identify kinases important for survival of CALR-mutant cells, we developed a novel strategy (KISMET) that
Ephrem G Kassa et al.
PLoS pathogens, 15(6), e1007851-e1007851 (2019-06-27)
Enteropathogenic E. coli (EPEC) is an extracellular diarrheagenic human pathogen which infects the apical plasma membrane of the small intestinal enterocytes. EPEC utilizes a type III secretion system to translocate bacterial effector proteins into its epithelial hosts. This activity, which
Eva Madi Riising et al.
PloS one, 3(7), e2704-e2704 (2008-07-17)
The Polycomb Repressive Complex 2 (PRC2) functions as a transcriptional repressor through a mechanism that involves methylation of Histone H3 at lysine 27. The PRC2 complex activity is essential for cellular proliferation, development, and cell fate decisions. PRC2 target genes
Articles
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Contenu apparenté
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Techniques, réactifs et protocoles permettant de purifier les protéines recombinantes par différentes méthodes telles que la chromatographie d'échange d'ions, la chromatographie d'exclusion stérique ou la chromatographie d'affinité.
Technologies d'expression protéique permettant d'exprimer des protéines recombinantes dans des systèmes d'expression de type E. coli, cellules d'insectes, levures et cellules de mammifères pour la recherche fondamentale et pour faciliter la production de médicaments et de vaccins.
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
-
Bonjour Je souhaiterais que vous me confirmiez que les billes Billes magnétiques Anti-FLAG® M2 sont bien directement couplées à l'Anticorps anti flag ? Pouvez vous également me fournir un protocole d'immunocapture utilisant ces billes s'il vous plait ?
1 réponse-
Utile ?
-
-
Which antibody is used to bind to the beads in Anti-FLAG® M2 Magnetic Beads(M8823) and EZview™ Red ANTI-FLAG® M2 Affinity Gel(F2426)? I have ordered the antibody(F1804 Monoclonal ANTI-FLAG® M2 antibody produced in mouse). Is it this one?
1 réponse-
The antibody used for these items is proprietary information. ANTI-FLAG M2 Magnetic Beads consist of a mouse-derived, ANTI-FLAG M2 monoclonal antibody attached to superparamagnetic iron-impregnated 4% agarose beads. EZview Red ANTI-FLAG M2 Affinity Gel contains ANTI-FLAG M2 monoclonal antibody covalently attached to cross-linked 4% agarose beads. The ANTI-FLAG M2 antibody recognizes the FLAG octapeptide sequence (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) at the N-terminus, Met-N-terminus, or C-terminus locations of a fusion protein.
Utile ?
-
-
Hello, I want to ask how many Flag-antibody on those beads?
1 réponse-
The specific number of antibodies per bead is not determined. The minimum amount of antibody in this product is 2.5 mg/mL of suspension. A range of 1.7 - 2.1 mL of suspension will yield 1 mL of packed resin. Both of these values are lot specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/m8823#product-documentationUtile ?
-
-
What is the maximum number of times that the ANTI-FLAG® M2 Magnetic Beads (M8823) can be reused?
What is the maximum number of times that the ANTI-FLAG® M2 Magnetic Beads (M8823) can be reused?
1 réponse-
The maximum number of times that the ANTI-FLAG® M2 Magnetic Beads (M8823) can be reused varies depending on the specific sample applied. It can range from one to twenty uses. Factors such as the cleanliness of the sample and the presence of substances that may clog the resin or cause nonspecific binding can affect the number of reuses. In typical scenarios, the beads are commonly reused for 3-5 times. It is essential to refer to the technical bulletin for specific situations where the beads cannot be reused, such as when using reagents containing SDS: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdf
Utile ?
-
-
Are the M2 antibodies on the Anti-FLAG® M2 Magnetic Beads covalently bound?
1 réponse-
The M2 Flag antibody is linked to the resin through a covalent linkage to one heavy chain. However, during the conjugation process, some Flag antibodies may also become bound to the resin through noncovalent attachments. To mitigate this, it is recommended to wash the resin using 0.1M Glycine followed by equilibration before using it. This washing step ensures that the noncovalently linked antibodies are washed off.
Utile ?
-
-
Is there a protocol available for using the Anti-Flag M2 Magnetic Beads in a ChIP-Seq assay? Additionally, if the lysis buffer contains SDS and sodium deoxycholate at a concentration of 0.1%, will the presence of both denature or interfere with binding in the research process?
1 réponse-
The use of Anti-Flag M2 Magnetic Beads in a ChIP-Seq assay has not been validated. However, according to a citation provided in Stem Cells (PMID: 31348575), it suggests that these beads are compatible for such applications. Please note that M8823 is not recommended for use with Sodium Dodecyl Sulfate (SDS) or deoxycholate, as these components can denature the immobilized antibody and disrupt its binding to FLAG fusion proteins.
Utile ?
-
-
Does M8823 have the capability to detect internal FLAG tags in addition to N and C terminal sequences?
1 réponse-
This paper (Zordan RE, Beliveau BJ, Trow JA, Craig NL, Cormack BP. Avoiding the ends: internal epitope tagging of proteins using transposon Tn7. Genetics. 2015 May;200(1):47-58. doi: 10.1534/genetics.114.169482. Epub 2015 Mar 5. PMID: 25745023; PMCID: PMC4423380) indicates that internal FLAG epiptopes were inserted into ORFs in yeast and detected by our F1804 antibody via Western blot. The biggest issue to overcome is the availability of the epitope for the antibody. If it is buried where it is not accessible in its native form, may not be possible. Denaturing the protein at least in this situation shows that the antibody can detect the protein.
Utile ?
-
-
Is Product No. CL4B200, Sepharose® CL-4B a suitable alternative for a negative bead control if magnetic beads without FLAG are not available? Are there any other recommendations for this purpose?
1 réponse-
For a negative control bead, any underivatized bead can essentially be used. While the Anti-FLAG® M2 Magnetic Beads (M8823) will work as a negative bead, it may not be suitable for magnetic applications. If a magnetic format is required, it is recommended to use polystyrene-based magnetized beads such as Product No. 49664, Micro particles based on polystyrene, magnetic. Alternatively, another option is to create a negative control using a non-FLAG tagged protein. This control can demonstrate the specificity of M8823 by showing its significant detection of FLAG-tagged proteins.
Utile ?
-
-
Are there any problems with incubation at 40°C for this product?
1 réponse-
The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
Utile ?
-
-
Dear all, I have a question regarding one of you product: Anti-FLAG® M2 Magnetic Beads, Cat. No: M8823. Is 0.1% SDS compatible with product binding FLAG peptide?
1 réponse-
This product is not recommended for use with Sodium Dodecyl sulfate nor deoxycholate as they are known to denature the immobilized antibody and interfere with antibody binding to FLAG fusion protein.
Regarding reagent compatibility and protocol information, please see page 5 of the product technical bulletin at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdfUtile ?
-
Filtres actifs
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
