71772
ProteoExtract® Transmembrane Protein Extraction Kit
Synonyme(s) :
Membrane Protein Isolation Kit
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About This Item
Code UNSPSC :
41106500
Nomenclature NACRES :
NA.77
Produits recommandés
Fabricant/nom de marque
Novagen®
Conditions de stockage
OK to freeze
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
Application
ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3) for extracting plasma membrane proteins
Caractéristiques et avantages
- No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
- Reagents are sufficient to process a total of 40 samples.
Avertissement
Toxicity: Toxic (F)
Autres remarques
Nakamura, E., et al. 2008. Cancer Sci.99, 1390.
Informations légales
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
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Hepatic Tmem30a Deficiency Causes Intrahepatic Cholestasis by Impairing Expression and Localization of Bile Salt Transporters
Liu L, et al.
The American Journal of Pathology, 187(12), 2775-2787 (2017)
Impairment of electroneutral Na+ transport and associated downregulation of NHE3 contributes to the development of diarrhea following in vivo challenge with Brachyspira spp.
Cole B. Enns, et al.
The American Journal of Physiology, 318(2), G288?G297-G288?G297 (2020)
Runtao Ding et al.
Frontiers in behavioral neuroscience, 14, 60-60 (2020-05-05)
The monocarboxylate transporters (MCTs) MCT1, MCT2, and MCT4 are essential components of the astrocyte-neuron lactate shuttle (ANLS), which is a fundamental element of brain energetics. Decreased expression of MCTs can induce cognitive dysfunction of the brain. In the present study
Huimin Luo et al.
Oncology reports, 42(3), 1237-1247 (2019-07-20)
Although cisplatin is one of the most accepted therapies for ovarian cancer, recurrence and drug resistance remain problematic. Both the ubiquitin‑proteasome system (UPS) and connexin (Cx) are closely related to tumor progression. However, the role of ubiquitin‑specific protease 14 (USP14) and Cx
Asahi Takeuchi et al.
Nutrients, 12(9) (2020-09-24)
IIAEK (Ile-Ile-Ala-Glu-Lys, lactostatin) is a novel cholesterol-lowering pentapeptide derived from bovine milk β-lactoglobulin. However, the molecular mechanisms underlying the IIAEK-mediated suppression of intestinal cholesterol absorption are unknown. Therefore, we evaluated the effects of IIAEK on intestinal cholesterol metabolism in a
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