T6258

Triosephosphate Isomerase from rabbit muscle

Type X, lyophilized powder, ≥3,500 units/mg protein

• Sinónimos: D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• Número de CAS: 9023-78-3
• Comisión internacional de enzimas: 5.3.1.1 (BRENDA, IUBMB)
• Número CE: 232-774-2
• Número MDL: MFCD00082080
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• tpo: Type X
• formulario: lyophilized powder
• actividad específica: ≥3,500 units/mg protein
• mol peso: calculated mol wt 53.2 kDa
• composición: Protein, 60-90% biuret
• actividad extraña: pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.

Aplicación

Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).

Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.

Acciones bioquímicas o fisiológicas

Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.

Envase

Package size based on protein content

Definición de unidad

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Forma física

Sulfate-free, contains EDTA and borate buffer salts

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)