A8811

Aldolase from rabbit muscle

ammonium sulfate suspension, 10-20 units/mg protein

• Sinónimos: D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase
• Número de CAS: 9024-52-6
• Comisión internacional de enzimas: 4.1.2.13 (BRENDA, IUBMB)
• Número MDL: MFCD00130453
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: rabbit muscle
• Nivel de calidad: 200
• Formulario: ammonium sulfate suspension
• actividad específica: 10-20 units/mg protein
• actividad extraña: glyceraldehyde-3-phosphate dehydrogenase ≤0.03%; lactic dehydrogenase ≤0.03%; phosphoglucose isomerase ≤0.6%; pyruvate kinase ≤0.1%; triosephosphate isomerase ≤0.05%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.[1]

Aplicación

Aldolase from rabbit muscle has been used:

• in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay[2]
• as a standard in the characterization of metabolic enzymes from glaucomatous tissues[3]
• in fructose 2,6-bisphosphate assay of human cell lines[4]

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 [5].

Acciones bioquímicas o fisiológicas

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics.[6] It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).[7]

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold [5].

Definición de unidad

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

Forma física

Crystalline suspension in 2.5 M (NH₄)₂SO₄, 0.01 M Tris, pH 7.5, 0.001 M EDTA

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable