T4528

Thioglucosidase from Sinapis alba (white mustard) seed

≥100 units/g solid

• Sinónimos: Glucosinolase, Myrosinase, Thioglucoside glucohydrolase
• Número CAS: 9025-38-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• Número CE: 3.2.1.147 (BRENDA, IUBMB)
• MDL number: MFCD00132422
• Specific activity: ≥100 units/g solid
• Biological source: plant seeds (Sinapis alba)

Propiedades

• biological source: plant seeds (Sinapis alba)
• Quality Segment: 100
• form: solid
• specific activity: ≥100 units/g solid
• storage temp.: −20°C

Descripción

General description

Thioglucosidase, also called as myrosinase, is present in the myrosin cells that do not contain glucosinolates. This enzyme is obtained from several plant sources, such as Lepidium sativum, L. Sinapis alba and Brassica napus.

Application

Thioglucosidase from Sinapis alba (white mustard) seeds has been used as a standard to quantify myrosinase activity and in column glucosinolate analysis of plant samples.

Thioglucosidase has been used in a study to assess Brassica species screening for glucosinolate content. Thioglucosidase has also been used in a study to investigate a negative regulatory role for auxin in sulphate deficiency response in Arabidopsis thaliana.

Biochem/physiol Actions

Thioglucosidase research has focused mainly on the cruciferous crops due to their economic importance and cancer preventive benefits.

Myrosinases are present in many bacteria, fungi, and edible plants, including those of the Brassicaceae (Cruciferae) family. The enzyme hydrolyzes the S-glucosidic bond of a glucosinolate substrate to form an unstable aglycone that rearranges with the loss of sulfate primarily to the isothiocyanate, though thiocyanates and nitriles are also formed. Many of the isothiocyanate products of aliphatic and aromatic glucosinolates have cancer chemopreventive properties.

Other Notes

One unit will produce 1.0 μmole glucose per min from sinigrin at pH 6.0 at 25 °C.

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)