This enzyme is a homodimer comprised of 27 kDa subunits.
T4330
Endonucleasas from Serratia marcescens
recombinant, expressed in E. coli
Sinónimos:
Endonuclease from Serratia marcescens
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50000 UNITS
323,00 €
323,00 €
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50000 UNITS
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
323,00 €
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origen biológico
Serratia marcescens
Nivel de calidad
recombinante
expressed in E. coli
Formulario
liquid
concentración
≥200,000 units/mL
técnicas
DNA purification: suitable
idoneidad
suitable for cell lysis
aplicaciones
life science and biopharma
temp. de almacenamiento
−20°C
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Descripción general
Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.[1]
Aplicación
Utilizado para eliminación de los ácidos nucleicos de las muestras proteicas.
Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification[2]. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.[3]
Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating. [4]
Acciones bioquímicas o fisiológicas
Digiere el ADN y el ARN nativos o desnaturalizados por calor.
Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing.[1]
Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.[5]
Definición de unidad
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Forma física
Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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M D Miller et al.
Journal of molecular biology, 288(5), 975-987 (1999-05-18)
Serratia endonuclease is an important member of a class of magnesium dependent nucleases that are widely distributed in nature. Here, we describe the location and geometry of a magnesium-water cluster within the active site of this enzyme. The sole protein
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