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Merck
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T1428

Sigma-Aldrich

apo-Transferrin bovine

BioReagent, suitable for cell culture, ≥98%

Sinónimos:

Human serotransferrin

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About This Item

Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.83

origen biológico

bovine

Línea del producto

BioReagent

Análisis

≥98%

formulario

powder

técnicas

cell culture | mammalian: suitable

impurezas

iron, essentially free

solubilidad

H2O: 10 mg/mL

Nº de acceso UniProt

temp. de almacenamiento

2-8°C

Información sobre el gen

bovine ... TF(280705)

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Descripción general

apo-Transferrin is an iron-free form of transferrin that is present in circulation.

Aplicación

All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred.
apo-Transferrin bovine has been used as a component of:
  • basal medium for culturing bovine mammary epithelial cells
  • minimum essential medium α (MEM-α) for spermatogonial stem cells (SSCs) culture
  • induction medium for mesenchymal stem cell culture

Acciones bioquímicas o fisiológicas

apo-Transferrin (apoTf) aids in preventing iron accumulation in tissues, especially in non-transfusion dependent β-thalassemia.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Shanshan Li et al.
Journal of animal science and biotechnology, 7, 44-44 (2016-08-06)
The objective of this study was to study how changing the ratio of Lys to Thr, Lys to His, and Lys to Val affects the expression of lipogenic genes and microRNA (miRNA) in bovine mammary epithelial cells. Triplicate cultures with
Jia Wang et al.
Stem cell research & therapy, 10(1), 227-227 (2019-07-31)
High temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still
Function and therapeutic development of apotransferrin.
Jaakko Parkkinen et al.
Vox sanguinis, 83 Suppl 1, 321-326 (2003-03-06)
Einar Vargas-Bello-Pérez et al.
Animal nutrition (Zhongguo xu mu shou yi xue hui), 5(2), 202-208 (2019-06-14)
The objective of this study was to determine how cytosolic triacylglycerols (TAG) are stored in mammary cells and whether this depends on the individual chemical configuration of fatty acids (FA). This objective was accomplished by addition of different FA to
Keita Abe et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 63(1-2), 154-156 (2008-04-05)
We have investigated the mechanism of manganese ion uptake by apo-transferrin using a capillary electrophoresis method, and obtained clear evidence that oxidation state +3 and the binuclear unit of a manganese chelate are essential factors for the facile uptake by

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