SRP0256
RB Active human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Sinónimos:
OSRC, RB1, Retinoblastoma 1, Retinoblastoma-associated protein, p105-Rb
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.32
Productos recomendados
origen biológico
human
recombinante
expressed in E. coli
Análisis
≥80% (SDS-PAGE)
formulario
aqueous solution
mol peso
43.5 kDa
envase
pkg of 200 μg
condiciones de almacenamiento
avoid repeated freeze/thaw cycles
concentración
>0.02 mg/mL
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Información sobre el gen
human ... RB1(5925)
Categorías relacionadas
Descripción general
The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Human recombinant RB protein (773-end) with N-terminal GST tag (GenBank Accession No. NM_000321), expressed in a E. coli expression system.
Aplicación
Retinoblastoma-associated protein (RB1) has been used in cell activity assay. It is also used as a kinase substrate for the kinase assay and in Western blotting.
Acciones bioquímicas o fisiológicas
Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma.
Forma física
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Nota de preparación
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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