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S100HR

Sigma-Aldrich

Sephacryl®

100-HR, MW range 1000-100,000 Da (globular proteins)

Sinónimos:

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
23151817
NACRES:
NA.56

descripción

stability: stable to 0.2M NaOH

formulario

suspension (20% aqueous ethanol)

Características

autoclavable

matriz

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

tamaño de partícula

25-75 μm (wet)
25-75 μm (wet)

tamaño de poro

1000-100,000 Da MW range (globular proteins)

compatibilidad

mode of use size exclusion chromatography

temp. de almacenamiento

2-8°C

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Descripción general

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)

Aplicación

Sephacryl® has been used:
  • to develop a novel high-throughput screening assay for sickle cell disease drug discovery
  • to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Información legal

Sephacryl is a registered trademark of Cytiva

Producto relacionado

Referencia del producto
Descripción
Precios

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Flam. Liq. 3

Código de clase de almacenamiento

3 - Flammable liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

100.4 - 109.4 °F - closed cup

Punto de inflamabilidad (°C)

38 - 43 °C - closed cup

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificados de análisis (COA)

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Diederik Johannes Opperman et al.
Journal of bacteriology, 190(8), 3076-3082 (2008-02-12)
Bacteria can reduce toxic and carcinogenic Cr(VI) to insoluble and less toxic Cr(III). Thermus scotoductus SA-01, a South African gold mine isolate, has been shown to be able to reduce a variety of metals, including Cr(VI). Here we report the
David Novak et al.
Bioelectrochemistry (Amsterdam, Netherlands), 119, 136-141 (2017-10-11)
Cytochrome c (cyt c) is one of the most studied conjugated proteins due to its electron-transfer properties and ability to regulate the processes involved in homeostasis or apoptosis. Here we report an electrochemical strategy for investigating the electroactivity of cyt
Matthew Lord et al.
The Journal of cell biology, 167(2), 315-325 (2004-10-27)
We purified native Myo2p/Cdc4p/Rlc1p (Myo2), the myosin-II motor required for cytokinesis by Schizosaccharomyces pombe. The Myo2p heavy chain associates with two light chains, Cdc4p and Rlc1p. Although crude Myo2 supported gliding motility of actin filaments in vitro, purified Myo2 lacked
Marc A Benson et al.
Clinica chimica acta; international journal of clinical chemistry, 411(3-4), 190-197 (2009-11-11)
Pseudomonas aeruginosa is an opportunistic pathogen that causes disease in immunocompromised individuals, burn victims, and cystic fibrosis patients. Strains that secrete ExoU induce host cell lysis and damage epithelial tissue, which can lead to severe outcomes including sepsis and mortality.
S A Kashani et al.
Biomaterials, artificial cells, and immobilization biotechnology : official journal of the International Society for Artificial Cells and Immobilization Biotechnology, 19(3), 579-598 (1991-01-01)
We previously reported that the cell free supernatant of hepatocyte cultures contained a hepatic stimulatory factor, which increases survival time and stimulates liver regeneration in the galactosamine induced fulminant hepatic failure animal model. When hepatocytes were microencapsulated in an alginate

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