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RAB0817

Sigma-Aldrich

Mouse Ins1 / Insulin-1 ELISA Kit

for serum, plasma and cell culture supernatants

Sinónimos:

Insulin

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About This Item

Código UNSPSC:
41116158
NACRES:
NA.32
En este momento no podemos mostrarle ni los precios ni la disponibilidad Póngase en contacto con el Servicio técnico

reactividad de especies

mouse

envase

kit of 96 wells (12 strips x 8 wells)

técnicas

ELISA: suitable

entrada

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 μIU/mL
standard curve range: 6.25-400 μIU/ml

método de detección

colorimetric

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

Información sobre el gen

mouse ... Ins1(16333)

Descripción general

The antibody pair provided in this kit recognizes Mouse Insulin 1.

Aplicación

Mouse Ins1 / Insulin-1 ELISA Kit has been used to measure changes in serum insulin in male Sprague-Dawley rats with chemically induced diabetes.[1]
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Acciones bioquímicas o fisiológicas

Certain organisms such as mice, rat and xenopus possess two non-allelic insulin genes. These genes are Ins1 and Ins2, which are both functional. They encode insulin that is produced in a 1:2 ratio, respectively in the pancreas. The two genes are differentially expressed in mouse. The Ins1 gene maps to mouse chromosome 19 in the telomeric region.[2] The insulin that is synthesized in the pancreas is crucial in maintaining glucose homeostasis. Knockout of Ins-1 and Ins-2 leads to insulin deficient mice with diabetes mellitus, ketoacidosis, liver steatosis and retarded growth.[3]

Otras notas

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pictogramas

Corrosion

Palabra de señalización

Warning

Frases de peligro

Consejos de prudencia

Clasificaciones de peligro

Met. Corr. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

David P Labbé et al.
Nature communications, 10(1), 4358-4358 (2019-09-27)
Systemic metabolic alterations associated with increased consumption of saturated fat and obesity are linked with increased risk of prostate cancer progression and mortality, but the molecular underpinnings of this association are poorly understood. Here, we demonstrate in a murine prostate
Differential expression and imprinting status of Ins1 and Ins2 genes in extraembryonic tissues of laboratory mice.
Deltour L, et al.
Gene expression patterns : GEP, 5(2), 297-300 (2004)
Phenotypic alterations in insulin-deficient mutant mice.
Duvillie B, et al.
Proceedings of the National Academy of Sciences of the USA, 94(10), 5137-5140 (1997)
Snehal N Chaudhari et al.
Nature chemical biology, 17(1), 20-29 (2020-08-05)
Bariatric surgery, the most effective treatment for obesity and type 2 diabetes, is associated with increased levels of the incretin hormone glucagon-like peptide-1 (GLP-1) and changes in levels of circulating bile acids. The levels of individual bile acids in the
Evaluation of White Sesame Seed Oil on Glucose Control and Biomarkers of Hepatic, Cardiac, and Renal Functions in Male Sprague-Dawley Rats with Chemically Induced Diabetes.
Aslam F, et al.
Journal of Medicinal Food, 20(5), 448-457 (2017)

Preguntas

1–2 de 2 Preguntas  
  1. What is the converting factor for rat and mouse insulin from IU/mL to ug/mL?

    1 respuesta
    1. The IU measurements and conversions are designated by the WHO. In this case, the WHO has not defined an IU for rat or mouse insulin, hence a conversion factor cannot be provided for this.

      ¿Le ha resultado útil?

  2. Can I please get a copy of the technical info/procedure for RAB0817 (Mouse Insulin ELISA)?

    1 respuesta
    1. The protocol for this product is provided in the lot-specific Certificate of Analysis.

      Please see the link below to review a sample Certificate:
      https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0817_1212J0534.pdf

      ¿Le ha resultado útil?

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