The IL-10 standard is prepared by diluting 5 uL with 728.3 uL of Assay Diluent A or Assay Diluent B. The formulations of both diluents are proprietary and may not be shared. Please see the link below for the sample Certificate of Analysis to review the protocol. Note the standard preparation instructions on page 2: https://www.sigmaaldrich.com/certificates/Graphics/COfAInfo/SigmaABNO/RAB0477_1013J0430.pdf
RAB0477
Mouse Tumor Necrosis Factor α ELISA Kit
for serum, plasma and cell culture supernatant
Sinónimos:
Cachectin, TNF-alpha, Tumor necrosis factor alpha
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NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Tnf(21926)
Input:
sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type cell culture supernatant(s)
sample type plasma
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice
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Servicio técnico
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Permítanos ayudarlespecies reactivity
mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type serum
sample type cell culture supernatant(s)
sample type plasma
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 93.75-6000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Tnf(21926)
General description
Tumor Necrosis Factor α (TNF-α) is a proinflammatory cytokine. It is a transmembrane protein and the final protein is devoid of the extracellular domain. TNF-α-converting enzyme (TACE) catalyzes the formation of the soluble homotrimer TNF-α.
The Mouse TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TNF-α in serum, plasma and cell culture supernatants.
Immunogen
Recombinant Mouse TNF-α
Application
Mouse Tumor Necrosis Factor α ELISA Kit has been used in measuring TNF-αin MC3T3-E1 murine preosteoblastic calvarial cell lines, rat serum, mice liver and kidney samples.
Biochem/physiol Actions
Tumor Necrosis Factor α (TNF-α) elicits inflammatory and immuno-regulatory functionality. It mediates its effects via its receptors TNFR1 and TNFR2.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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Este artículo | |||
|---|---|---|---|
| Gene Information mouse ... Tnf(21926) | Gene Information rat ... Tnf(24835) | Gene Information human ... LTA(4049) | Gene Information Porcine ... TNF(397086) |
| species reactivity mouse | species reactivity rat | species reactivity human | species reactivity porcine |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type serum | input sample type serum | input sample type cell culture supernatant(s) | input sample type serum |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS & Precios
signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Clase de almacenamiento
10 - Combustible liquids
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How to prepare lyophilized reagents from ELISA kits for IL10 elisa sigma RAB 0244-1KT and on standard dilution: 5microl + 728.3 microl BUT WHAT IS 728.3 microl
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