M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

• Sinónimos: Moloney Murine Leukemia Virus Reverse Transcriptase
• Número de CAS: 9068-38-6
• Número MDL: MFCD00283754
• Código UNSPSC: 12352202
• NACRES: NA.55

Propiedades

• origen biológico: Porcine intestinal mucosa
• Nivel de calidad: 200
• recombinante: expressed in E. coli
• Formulario: liquid
• uso: sufficient for 200 reactions; sufficient for 250 reactions
• Características: dNTPs included: no; hotstart: no
• concentración: 200 units/μL
• técnicas: RT-PCR: suitable
• color: colorless
• entrada: purified RNA
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Aplicación

M-MLV Reverse Transcriptase has been used:

• for the preparation of cDNA libraries or for first strand cDNA synthesis
• for use in a 2-step RT-PCR assay
• in quantitative realtime-polymerase chain reaction (RT-qPCR)
• in reverse transcription

Características y beneficios

• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.

Envase

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Definición de unidad

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Nota de preparación

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Información legal

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)