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Merck

P5726

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 2

aqueous solution (dark coloration may develop upon storage, which does not affect the activity), for the inhibition of tyrosine protein phosphatases, acid and alkaline phosphatases

Sinónimos:

Protease inhibitor

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.54

product name

Phosphatase Inhibitor Cocktail 2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

formulario

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

Nivel de calidad

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Phosphatase Inhibitor Cocktail 2 is a mixture of components designed to inhibit enzymes that modify proteins present in cell extracts, leading to improved yields of intact proteins. It has been optimized and tested for use on various animal tissues and cytosolic extracts.

Aplicación

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.

Acciones bioquímicas o fisiológicas

This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.

Características y beneficios

This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Precaución

As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.

Nota de preparación

This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.

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Pictogramas

Health hazardExclamation mark

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 2


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

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