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Merck

P2850

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 1

DMSO solution, mixture of inhibitors

Sinónimos:

Phosphatase Inhibitor

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About This Item

Número CE:
Código UNSPSC:
12352200
NACRES:
NA.54

Nombre del producto

Phosphatase Inhibitor Cocktail 1, DMSO solution

Formulario

DMSO solution

Nivel de calidad

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

Aplicación

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation. It has also been used in western blot analysis.

Acciones bioquímicas o fisiológicas

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.

Cantidad

Recommended usage is a 100-fold dilution.

Nota de análisis

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

188.6 °F

Punto de inflamabilidad (°C)

87 °C


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X Zhao et al.
Mucosal immunology, 10(6), 1553-1568 (2017-04-13)
Virulent Mycobacterium tuberculosis (Mtb) triggers necrosis in host Mϕ, which is essential for successful pathogenesis in tuberculosis. Here we demonstrate that necrosis of Mtb-infected Mϕ is dependent on the action of the cytosolic Receptor Interacting Protein Kinase 3 (RIPK3) and
Class III β-tubulin expression and in vitro resistance to microtubule targeting agents.
Stengel C, et al.
British Journal of Cancer, 102(2), 316-316 (2010)
Larger therapeutic window for steroid versus VEGF-A inhibitor in inflammatory angiogenesis: surprisingly similar impact on leukocyte infiltration.
Nakao S, et al.
Investigative Ophthalmology & Visual Science, 53(7), 3296-3302 (2012)
Discovery of protein phosphatase inhibitor classes by biology-oriented synthesis.
Noren M A, et al.
Proceedings of the National Academy of Sciences of the USA, 103(28), 10606-10611 (2006)
Aberrant Wnt/β-catenin signaling in pancreatic adenocarcinoma.
Zeng G, et al.
Neoplasia, 8(4), 279-289 (2006)

Artículos

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

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