P3397

Phosphoglucomutase from rabbit muscle

ammonium sulfate suspension, ≥100 units/mg protein

• Sinónimos: PGM, α-D-Glucose-1,6-bisphosphatase, α-D-Glucose-1-phosphate phosphotransferase
• Número de CAS: 9001-81-4
• Comisión internacional de enzimas: 5.4.2.2 (BRENDA, IUBMB)
• Número MDL: MFCD00131886
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: rabbit muscle
• descripción: ammonium sulfate suspension, >=100 units/mg protein
• formulario: ammonium sulfate suspension
• actividad específica: ≥100 units/mg protein
• condiciones de almacenamiento: (Tightly closed)
• técnicas: activity assay: suitable
• color: white to light yellow
• actividad extraña: lactic dehydrogenase ≤0.5%; phosphoglucose isomerase ≤0.01%; pyruvate kinase ≤0.05%
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Research area: Cell Signaling

Phosphoglucomutase (PGM), a conserved enzyme, is abundantly found in animals, plants, and microorganisms. It is distributed in almost all tissues.

Aplicación

Phosphoglycomutase has been used:

• to study glycogenesis and phosphoglycomutase deficiency in humans
• in phosphoglucomutase assays to study metabolic regulation
• for assay A to assay enzymes closely linked to glycogen metabolism in transformed BL21(DE3)C43 cells
• in sucrose synthase (SuSy) andADPglucose pyrophosphorylase (AGPase) assays(3)
• in enzyme activity assay for coupling the reduction of nicotinamide adenine dinucleotide phosphate (NADP⁺)to determine glucose-1-phosphate from sucrose and inorganic phosphate (Pi)

Acciones bioquímicas o fisiológicas

Phosphoglucomutase(PGM) enzyme plays a vital role in glycolysis and gluconeogenesis. It is involved in glycogen and trehalose metabolism in insects. PGM participates in the development of plants and some microorganisms. It is essential for proteins, lipids, and nucleic acid metabolism and is crucial for the development of plants because glucose-6-phosphate is a crucial central metabolite. PGM catalyzes the interconversion of glucose-6-phosphate (G-6-P)and glucose-1-phosphate (G-1-P).

Definición de unidad

One unit will convert 1.0 μmole of α-D-glucose 1-phosphate to α-D-glucose 6-phosphate per min at pH 7.4 at 30 °C.

Forma física

Crystalline suspension in 3.2 M (NH₄)₂SO₄, pH 6.0 containing 0.01% EDTA

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)