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To collect the agarose beads, a centrifugation speed of 1000 x g for 5 minutes should be sufficient, using the A2220 batch method as a guide
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
Sinónimos:
Alkaline phosphatase-Agarose
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50 UNITS
279,00 €
100 UNITS
479,00 €
250 UNITS
1030,00 €
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3250,00 €
279,00 €
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50 UNITS
279,00 €
100 UNITS
479,00 €
250 UNITS
1030,00 €
1000 UNITS
3250,00 €
About This Item
279,00 €
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Productos recomendados
origen biológico
bovine (calf) intestine
Nivel de calidad
Formulario
ammonium sulfate suspension
actividad específica
250-1000 units/mL packed gel
Matriz
beaded agarose
temp. de almacenamiento
2-8°C
Categorías relacionadas
Descripción general
Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. [1]
Aplicación
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. [2]
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. [3] It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases. [4]
Acciones bioquímicas o fisiológicas
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
Envase
Package size based on DEA units
Definición de unidad
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
Forma física
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Listados normativos
Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.
EU REACH Annex XVII (Restriction List)
CAS No.
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1. What is the recommended speed for spinning down the phosphatase, alkaline-agarose when using this product?
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