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P4252

Phosphatase, Alkaline from Escherichia coli

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Sinónimos:

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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Talla/SKUDisponibilidadPrecio
100 units
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
331,00 €
200 units
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
1340,00 €
1000 units
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
1910,00 €
1623,50 €

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Número CAS:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
MFCD00131849
Número CE:
3.1.3.1 (BRENDA, IUBMB)
Specific activity:
30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
Biological source:
Escherichia coli

331,00 €

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biological source

Escherichia coli

Quality Segment

form

ammonium sulfate suspension

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

technique(s)

protein detection: suitable

storage temp.

2-8°C

Application

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.

Biochem/physiol Actions

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Physical form

Suspension in 2.5 M (NH4)2SO4

Analysis Note

The activity at pH 8.0 at 25 °C is approximately the same as at pH 10.4 at 37 °C.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.

Disclaimer

Presence of 0.1% SDS in the alkaline phosphatase assays will inhibit any nuclease contamination in the sample.

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Este artículo
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Phosphatase, Alkaline from Escherichia coli ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

Sigma-Aldrich

P4252

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from Escherichia coli lyophilized powder, 30-60 units/mg protein (in glycine buffer)

Sigma-Aldrich

P5931

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from Escherichia coli buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

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P4069

Phosphatase, Alkaline from Escherichia coli

Phosphatase, Alkaline from porcine kidney lyophilized powder, ≥100 DEA units/mg protein

Sigma-Aldrich

P4439

Phosphatase, Alkaline from porcine kidney

description

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

description

lyophilized powder, 30-60 units/mg protein (in glycine buffer)

description

buffered aqueous glycerol solution, 20-50 units/mg protein (in glycine buffer)

description

lyophilized powder, ≥100 DEA units/mg protein

biological source

Escherichia coli

biological source

-

biological source

Escherichia coli

biological source

-

specific activity

30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

specific activity

30-60 units/mg protein (in glycine buffer)

specific activity

20-50 units/mg protein (in glycine buffer)

specific activity

≥100 DEA units/mg protein

form

ammonium sulfate suspension

form

lyophilized powder

form

buffered aqueous glycerol solution

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

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Pictogramas

Health hazard
GHS08

Palabra de advertencia

Danger

Códigos de peligro

H334

Indicaciones de precaución

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

¿Qué está pasando?

WGK 1

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

EPI (equipos de protección individual)

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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