Product No. MAK107 comes with a "Substrate Reagent" component which is used as the substrate in this assay.
MAK107
LCAT Activity Assay Kit
Supplied by Roar Biomedical, Inc.
Sinónimos:
Lecithin:cholesterol acyltransferase activity assay kit
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About This Item
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uso
sufficient for 100 fluorometric tests
aplicaciones
pharmaceutical
método de detección
fluorometric
enfermedades relevantes
cardiovascular diseases
temp. de almacenamiento
−20°C
Información sobre el gen
human ... LCAT(3931)
Descripción general
The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.
Aplicación
Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
Características y beneficios
Compatible with high-throughput handling systems.
Idoneidad
Suitable for measuring phospholipase activity of LCAT in plasma or serum.
Principio
The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.
Solo componentes del kit
Referencia del producto
Descripción
- Substrate Reagent .1 mL
- READ Reagent 30 mL
- LCAT Assay Buffer 20 mL
Producto relacionado
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Código de clase de almacenamiento
3 - Flammable liquids
Punto de inflamabilidad (°F)
49.5 °F
Punto de inflamabilidad (°C)
9.7 °C
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What substrate should be used to create the standard curve for this assay?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Are products MAK107 and MAK108 compatible with mouse serum?
1 respuesta-
Yes, but please be aware of possible sample hemolysis. Depending on the method of mouse sample collection, hemolysis may be common, which can interfere with fluorescence emission via color quenching.
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