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Merck

MAK082

Sigma-Aldrich

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Sinónimos:

ALDH Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detección

colorimetric

enfermedades relevantes

cardiovascular diseases; cancer; hematological disorder

temp. de almacenamiento

−20°C

Descripción general

Aldehyde dehydrogenases (ALDH) are a family of enzymes that catalyze the metabolism of exogenous and endogenous aldehydes, preventing the accumulation of potentially reactive and toxic aldehydes and their metabolites. In addition to their role in aldehyde metabolism, ALDH enzymes also play critical roles in other cellular processes such as cell proliferation, differentiation, and survival.[1][2] Mutations in ALDH family members have been linked to numerous diseases such as heart disease, cataracts, and certain cancers. ALDH activity may also serve as a marker for certain stem cell populations including hematopoietic stem cells and certain cancer stem cells.[3]

Aplicación

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the activity of aldehyde dehydrogenase.[4][5]

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for the measurement of aldehyde dehydrogenase (ALDH) in a variety of samples including tissue and cells

Principio

The Aldehyde Dehydrogenase Activity Colorimetric Assay kit provides a simple and direct procedure for measuring NAD-dependent ALDH activity in a variety of samples. ALDH activity is determined by a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the ALDH activity present. One unit of ALDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Pictogramas

Health hazardCorrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Carc. 1B - Eye Dam. 1 - Skin Corr. 1B

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 8(2), 2002232-2002232 (2021-01-30)
Cancer stem cells (CSCs) are considered the roots of cancer metastasis and recurrence (CSCs), due in part to their self-renewal and therapy resistance properties. However, the underlying mechanisms for the regulation of CSC stemness are poorly understood. Recently, increasing evidence
Xiaotian Xu et al.
Aging, 14(11), 4858-4873 (2022-06-10)
The roles of asparagine-linked glycosylation (ALG) members in tumorigenic process have been widely explored. However, their effects in colorectal cancer progression are still confusing. Here, we screened 12 ALGs' expression through online datasets and found that ALG10 was mostly upregulated
A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.
Yilu Qin et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(24), e2204046-e2204046 (2022-08-26)
Characterization of the CD49f+/CD44+/CD24- single-cell derived stem cell population in basal-like DCIS cells.
Duru N, et al.
Oncotarget, 7(30), 47511-47511 (2016)
Ting Liu et al.
Bioengineered, 12(1), 7143-7155 (2021-09-22)
Cancer stem cell (CSC) has been confirmed to trigger tumor occurrence and progression and CSC can develop strategies to maintain a lower reactive oxygen species (ROS) level compared to cancer cells. However, the mechanisms contributing to ROS homeostasis in CSC

Artículos

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

Preguntas

1–3 de 3 Preguntas  
  1. Is it possible for the assay buffer to lyse the cells without the use of additional instrumentation such as a sonicator?

    1 respuesta
    1. It is recommended to use a dounce homogenizer on ice or perform mild sonication on ice to lyse the cells. The assay buffer contains detergent but will not lyse the cells by simply incubating in it.

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  2. Is there a general range expected for the activity of the ALDH positive control? Given variances in room temperature, etc.

    1 respuesta
    1. The positive control does not have an expected activity range.

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  3. What is the kit Sensitivity? thanks,

    1 respuesta
    1. This assay kit can detect < 0.5 mU/well of ALDH activity (based on the unit definition) in a variety of samples. Unit Definition: One unit is the amount of enzyme that will generate 1.0 umol of NADH per min at pH 8 at room temperature. This is a sensitive assay for measuring ALDH activity in various biological samples and the kit is also suitable for high throughput analyses.

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