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M0535

Sigma-Aldrich

Mycoplasma Broth

powder, suitable for microbiology

Sinónimos:

Mycoplasma media

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250 G
393,00 €
500 G
687,00 €

393,00 €

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250 G
393,00 €
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687,00 €

About This Item

Código UNSPSC:
41106200
NACRES:
NA.74

393,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

Formulario

powder

Nivel de calidad

200

técnicas

microbiological culture: suitable

solubilidad

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

aplicaciones

microbiology

Descripción general

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Aplicación

Mycoplasma Broth has been used for isolation and cultivation of mycoplasma.[1] It may also be used to grow Porphyromonas gingivalis under anerobic conditions.[2]

Componentes

Powder contains (g/L): 10.0 bacterial peptone, 10.0 LAB-LEMCO powder, 5.0 sodium chloride, 0.5 mineral supplement.

Nota de preparación

Basal Medium:

1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.

2. Adjust the pH of the solution to 7.8 ± 0.2.

3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.

Almacenamiento y estabilidad

Store dry powdered medium at room temperature and prepare medium at 2-8 °C.

Otras notas

The product is intended for laboratory use only.

Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000386626
0000351648
0000334603
0000318192
0000318192

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Artículos

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Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

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Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Good Cell Banking Practices

Overview of good cell banking practices for cell line cryopreservation purposes.

Quality Control Considerations in Cell Culture

Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

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