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Merck

MP0030

Sigma-Aldrich

LookOut® Mycoplasma Elimination Kit

Sinónimos:

Mycoplasma contamination treatment

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1 KIT
345,00 €

345,00 €


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1 KIT
345,00 €

About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

345,00 €


Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

uso

 kit sufficient for 5 mL mycoplasma elimination

Nivel de calidad

envase

pkg of 1 kit

condiciones de almacenamiento

protect from light

técnicas

PCR: suitable

aplicaciones

detection
microbiology

temp. de almacenamiento

2-8°C

Descripción general

The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.

Aplicación

The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.

Características y beneficios

  • Minimal cytotoxic properties of the reagents
  • Highly effective
  • Retention of normal cell characteristics

Idoneidad

The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.

Información legal

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.

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Código de clase de almacenamiento

10 - Combustible liquids


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Certificados de análisis (COA)

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Visite la Librería de documentos

Optimized PCR-based Detection of Mycoplasma
Dobrovolny PL and Bess D
Journal of Visualized Experiments, 52 (2011)

Artículos

Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.

Detect mycoplasma contamination in cell culture through the PCR, DNA stain, or culture tests. Discover mycoplasma prevention, elimination, and detection kits.

Overview on how to maintain proper cell culture laboratory safety including steps on performing lab risk assessments, surface disinfection, biohazard and waste removal. Free ECACC handbook download.

Overview of good cell banking practices for cell line cryopreservation purposes.

Ver todo

Protocolos

Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.

Preguntas

1–2 de 2 Preguntas  
  1. What antibiotics are included in MP0030, the LookOut® Mycoplasma Elimination Kit? Are fluoroquinolones, macrolides, or tetracyclines part of the kit?

    1 respuesta
    1. The MP0030 product contains two mycoplasma-active antibiotics, with one of them being a fluoroquinolone.

      ¿Le ha resultado útil?

  2. How does the mechanism of action (MOA) of Product No. MP0030, LookOut® Mycoplasma Elimination Kit, differ from that of BM cyclin?

    1 respuesta
    1. The key distinction lies in Product No. MP0030, LookOut® Mycoplasma Elimination Kit, which actively eradicates mycoplasma by disrupting the membrane. As a lipopeptide, it identifies cholesterol-rich regions in the membrane (as the concentration of cholesterol in mycoplasma membranes is higher than in eukaryotic cells), creating a pore through which the surrounding medium flows into the mycoplasma, ultimately causing it to burst. In contrast, standard antibiotics simply inhibit metabolic pathways, necessitating the rinsing out of mycoplasma through a medium change. If the process is halted prematurely, any remaining mycoplasma can regrow.

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