M042030

Biotin Phosphoramidite

configured for ABI

• Sinónimos: Biotin amidite
• Fórmula empírica (notación de Hill): C₄₆H₆₄N₅O₈PS
• Número de CAS: 1217500-22-5
• Peso molecular: 878.07
• Número MDL: MFCD00676535
• Código UNSPSC: 41116105
• ID de la sustancia en PubChem: 329817780
• NACRES: NA.21

Propiedades

• Línea del producto: Proligo Reagents
• Ensayo: ≥96% (³¹P-NMR)
• idoneidad: conforms to structure for H-NMR
• compatibilidad: configured for ABI
• temp. de almacenamiento: −20°C
• cadena SMILES: COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5
• InChI: 1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1
• Clave InChI: WSDQYLGSIUJJRU-ZMKGMYJVSA-N

Descripción

Descripción general

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis. Biotin-labelled oligonucleotides have become extremely popular in DNAcapture and detection applications, due to their very high-sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotinbased detection methods and are widely used by researchers. Biotin labelling is compatible with PCR and most hybridization techniques.

Key Features of Biotin Phosphoramidite

• Behaves like any standard phosphoramidite on the DNA synthesizer
• Readily soluble in acetonitrile
• Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification
• A long, water-compatible linker arm ensures maximum sensitivity
• Provides a coupling efficiency of at least 95%The product is configured for ABI^™ Synthesizers

Información legal

ABI is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable