Saltar al contenido
Merck

Documentos clave

Ver Toda la documentación

Saltar a

L4144

Lectina de Phaseolus vulgaris (frijol rojo)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Sinónimos:

Aglutinina de Phaseolus vulgaris, PHA

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
A ustedes/SKUDisponibilidadPrecio
5 mg
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
399,00 €

Acerca de este artículo

UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Form:
lyophilized powder

399,00 €

Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
​
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle

Quality Segment

200

product line

BioReagent

form

lyophilized powder

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.), ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

suitability

leucocyte agglutination activity tested

storage temp.

2-8°C

General description

Lectins are carbohydrate-binding proteins, which contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • it has been used as a positive control to treat cells
  • to stimulate cell culture for the analysis of lymphocyte proliferation
  • to stimulate T cells

Biochem/physiol Actions

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.
La PHA consta de dos especies moleculares, la eritroaglutinina (PHA-E), que tiene poca actividad mitógena y elevada actividad de eritroaglutinina, y la leoucoaglutinina (PHA-L), que tiene elevada actividad mitógena y leoucoaglutinante, pero muy baja actividad eritroaglutininante
La PHA-E no es específica de grupo sanguíneo, pero la aglutinación puede ser inhibida por ciertos oligosacáridos. La PHA-P es la forma proteica de la PHA antes de la separación y la purificación de la eritroaglutinina y la leoucoaglutinina. La PHA-M es la forma mucoproteínica. Los conjugados se preparan a partir de las correspondientes lectinas purificadas.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
L8754L264661764
Lectina de Phaseolus vulgaris (frijol rojo) Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

L4144

Lectina de Phaseolus vulgaris (frijol rojo)

Lectina de Phaseolus vulgaris (frijol rojo) Phytohemagglutinin PHA-P, lyophilized powder

Sigma-Aldrich

L8754

Lectina de Phaseolus vulgaris (frijol rojo)

Lectina de Phaseolus vulgaris (frijol rojo) Phytohemagglutinin PHA-M, lyophilized powder

Sigma-Aldrich

L2646

Lectina de Phaseolus vulgaris (frijol rojo)

Sigma-Aldrich

Sigma-Aldrich

61764

Lectin from Phaseolus vulgaris

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

tissue culture: suitable

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

powder

suitability

leucocyte agglutination activity tested

suitability

-

suitability

-

suitability

-

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

solubility

-

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

impurities

salt, free

impurities

salt, free

impurities

salt, free

impurities

-

Still not finding the right product?

Explore all of our products under Lectina de Phaseolus vulgaris (frijol rojo)

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Preguntas

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 respuesta

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      ¿Le ha resultado útil?

Revisiones

Sin puntuación

Filtros activos