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I8896

Sigma-Aldrich

IGEPAL® CA-630

for molecular biology

Sinónimos:

Octylphenoxy poly(ethyleneoxy)ethanol, branched

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About This Item

Fórmula lineal:
(C2H4O)nC14H22O
Número de CAS:
9002-93-1
Código UNSPSC:
12352104
NACRES:
NA.31

109,00 €

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grado

for molecular biology

Nivel de calidad

200

descripción

non-ionic

Formulario

liquid

técnicas

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

HLB

13

actividad extraña

DNase and RNase, none detected

cadena SMILES

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

Clave InChI

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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Descripción general

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.[1]

Aplicación

IGEPAL® CA-630 has been used
  • as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates[2]
  • In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos[3]
  • In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates[4]

Otras notas

Nonionic, non-denaturing detergent

Calidad

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Precaución

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Información legal

IGEPAL is a registered trademark of Solvay

Palabra de señalización

Danger

Frases de peligro

H302,H315,H318,H410

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

483.8 °F - closed cup

Punto de inflamabilidad (°C)

251 °C - closed cup

Equipo de protección personal

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH SVHC Candidate List

CAS No.

EU REACH Annex XIV (Authorisation List)

CAS No.

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
MKCW7585
MKCV4219
MKCR5566
MKCR3234
MKCS4114

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Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
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Protocolos

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Contenido relacionado

Técnicas de análisis pull-down de proteínas

Investigue las interacciones proteína-proteína in vitro con análisis pull-down, utilizando métodos de afinidad, pull-down con GST, TAP y co-inmunoprecipitación.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Preguntas

1–10 de 17 Preguntas  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      ¿Le ha resultado útil?

  3. In which solvent(s) is Atazanavir Sulfate soluble?

    1 respuesta

    1. Atazanavir Sulfate is soluble in DMSO: 104mg/mL and EtOH: 20mg/mL.

      ¿Le ha resultado útil?

  4. What is the storage temperature for this item?

    1 respuesta

    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).

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  5. Hi, I am enquiring about the storage temperature of product IGEPAL(R) CA-630 (I8896-100ml). The storage class is listed in the SDS but no temperature upper and lower limits. Would I be able to request this information. Thank you

    1 respuesta

    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, and the Safety Data Sheet (SDS).

      ¿Le ha resultado útil?

  6. What is the molecular weight of Product No. I8896, IGEPAL® CA-630?

    1 respuesta

    1. The approximate molecular weight of this product is 603 Daltons. The average EO (ethylene oxide) units per molecule are 9.

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  7. What is the wt/vol % of this IGEPAL reagent? I want to use it for ATAC-seq and in the protocols people always mention the concentration of the stock is 10% wt/vol but I can't find this information on the specifications of this product. Thanks

    1 respuesta

    1. This product is provided as a neat liquid with a concentration of 100% which can be further diluted. The density of IGEPAL CA-630 is 1.06 g/cm3 at 25 °C. For a 10% wt./vol. solution 9.43 mL IGEPAL is needed per 100 mL of solution.

      The product is generally stable based on the chemical composition and a 10% (w/v) aqueous solution may be stable refrigerated for at least one month. However, it is important to minimize the exposure of the solution to oxygen (keep closed soon after use). The product does have a tendency to oxidize in air forming peroxides through a free radical process. In addition, some bacterial growth may occur which is evidenced by an odor or formation of a precipitate. Preservatives added to the solution may prevent bacterial growth. Please view the data sheet below: https://www.braskem.com.br/Portal/Principal/Arquivos/html/boletm_tecnico/PP%20Chemical%20Resistance.pdf

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  8. What is the viscosity of Product No. I8896, IGEPAL® CA-630?

    1 respuesta

    1. The viscosity is determined for each lot and the information is available on the lot- specific Certificate of Analysis.

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  9. Are the 2 products, Igepal CA-630 (Product No. I8896) and Nonidet 40 the same? How do you use this product?

    1 respuesta

    1. Igepal CA-630 is chemically indistinguishable to NP40. This non-denaturing detergent is used the same way as that of NP40.

      ¿Le ha resultado útil?

  10. What does "Molecular Biology Grade" mean?

    1 respuesta

    1. Molecular Biology Grade means that this product must meet the DNase, RNase, and Nickase specification on the Certificate of Analysis for each lot of the product.

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1–10 de 17 Preguntas  

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